D-glucarate, 5-keto-4-D-deoxyglucarate and α-ketoglutarate semialdehyde were separated by HPLC, using an Ultimate-3000 HPLC system (Dionex, Idstein, Germany), equipped with an autosampler (WPS 3000TRS), a column compartment (TCC3000RS) and a diode array detector (DAD 3000RS). The column Metrosep A Supp10–250/40 column (250 mm, particle size 4.6 mm; Metrohm, Filderstadt, Germany) at 65 °C was used for separation by isocratic elution with 30 mM ammonium bicarbonate (pH 10.4) as the mobile phase at 0.2 mL min−1. Samples were diluted in water, filtered (10 kDa MWCO, modified PES; VWR, Darmstadt, Germany) and 10 μL of the samples was applied on the column. Data was analysed with Dionex Chromelion software.
Chromelion software
Manufactured by Thermo Fisher Scientific
Sourced in United States
Chromelion software is a powerful data acquisition and processing solution for liquid chromatography (LC) and ion chromatography (IC) systems. It provides a comprehensive platform for instrument control, data collection, and data analysis.
Automatically generated - may contain errors
Lab products found in correlation
Metrosep a supp10 250 40 column, by Metrohm (1 mentions)
Dad 3000rs, by Thermo Fisher Scientific (1 mentions)
Tcc 3000rs, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 hplc system, by Thermo Fisher Scientific (1 mentions)
Wps 3000trs, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 rs column, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 rs autosampler, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 rs pump, by Thermo Fisher Scientific (1 mentions)
2 protocols using chromelion software
1
HPLC Separation of Glucarate Metabolites
2
HPLC Quantification of Charantin in WBG
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The WBG extract was dissolved in 25 mg/ml methanol and filtered through
a 0.45 µl membrane filter for HPLC analysis. The HPLC system was a Dionex
UltiMate 3000 HPLC system, which consisted of an UltiMate 3000 RS pump, an UltiMate 3000
RS autosampler, an UltiMate 3000 RS column compartment, a variable wavelength detector
(Dionex, Olten, Switzerland) and a reversed-phase column (Inertsil ODS-3V, 4.6 Â, 150 mm,
GL Science, Inc., Tokyo, Japan). Each charantin standard and all samples were prepared in
injection volumes of 20 µl with detection at a wavelength of 204 nm and a
column flow rate of 1 ml, and the mobile phase was methanol-water 100:2
(V/V). Data were collected and stored in the Chromelion software from Dionex Corporation
(Sunnyvale, CA, U.S.A.).
a 0.45 µl membrane filter for HPLC analysis. The HPLC system was a Dionex
UltiMate 3000 HPLC system, which consisted of an UltiMate 3000 RS pump, an UltiMate 3000
RS autosampler, an UltiMate 3000 RS column compartment, a variable wavelength detector
(Dionex, Olten, Switzerland) and a reversed-phase column (Inertsil ODS-3V, 4.6 Â, 150 mm,
GL Science, Inc., Tokyo, Japan). Each charantin standard and all samples were prepared in
injection volumes of 20 µl with detection at a wavelength of 204 nm and a
column flow rate of 1 ml, and the mobile phase was methanol-water 100:2
(V/V). Data were collected and stored in the Chromelion software from Dionex Corporation
(Sunnyvale, CA, U.S.A.).
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