Rabbit anti rad18

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-RAD18 is a primary antibody that specifically recognizes the RAD18 protein. RAD18 is an E3 ubiquitin ligase that plays a role in DNA damage response and post-replication repair. The antibody can be used to detect the expression and localization of RAD18 in various experimental systems.

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Lab products found in correlation

Rabbit anti ubiquityl pcna lys164, by Cell Signaling Technology (2 mentions) Rabbit anti polh, by Cell Signaling Technology (2 mentions) Rabbit anti cyld, by Cell Signaling Technology (2 mentions) Mouse anti pcna pc10, by Santa Cruz Biotechnology (2 mentions) Irdye 800cw goat anti mouse igg, by LI COR (2 mentions) Mouse anti gapdh, by Merck Group (2 mentions) Irdye 800cw goat anti rabbit igg, by LI COR (2 mentions) Irdye 680rd goat anti rabbit igg, by LI COR (2 mentions) Irdye 680rd goat anti mouse igg, by LI COR (2 mentions) Mouse anti flag m2, by Merck Group (2 mentions) Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Rad18 (2 citations)

2 protocols using rabbit anti rad18

Antibody Characterization for Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercially available antibodies used were as follows: rabbit anti-RAD18 (Cell Signaling, Danvers, MA, USA, 9040S, dilution 1:2000); rabbit anti-ubiquityl-PCNA (Lys164) (Cell Signaling, 13439S, dilution 1:2000); rabbit anti-GAPDH (Cell Signaling, 5174S, dilution 1:10000); rabbit anti-POLH (Cell Signaling, 13848S, dilution 1:1000); rabbit anti-CYLD (Cell Signaling, 8462S, dilution 1:1000); mouse anti-GAPDH (EMD Millipore Corporation, Temecula, CA, USA, MAB374, dilution 1:10000); mouse anti-PCNA (PC-10) (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-56, dilution 1:1000); rabbit anti-TRF4 (H-172) (TENT4A) (Santa Cruz Biotechnology, sc-98490, dilution 1:500); mouse anti-FLAG M2 (Sigma-Aldrich, F1804, dilution 1:1000); rabbit anti-PTIP (PAXIP1) (Bethyl Laboratories, Montgomery, TX, USA, A300–370A, dilution 1:2000); rabbit anti-DTL (Bethyl Laboratories, A300–948A, dilution 1:1000); anti-MBP monoclonal antibody, HRP conjugated (New England Biolabs, Ipswich, MA, USA, E8038S, dilution 1:10000); IRDye 680RD goat anti-mouse IgG (LI-COR Biosciences, 926–68070, dilution 1:10000); IRDye 800CW goat anti-mouse IgG (LI-COR Biosciences, 926–32210, dilution 1:10000); IRDye 680RD goat anti-rabbit IgG (LI-COR Biosciences, 926–68071, dilution 1:10000); IRDye 800CW goat anti-rabbit IgG (LI-COR Biosciences, 926–32211, dilution 1:10000).

Swain U., Friedlander G., Sehrawat U., Sarusi-Portuguez A., Rotkopf R., Ebert C., Paz-Elizur T., Dikstein R., Carell T., Geacintov N.E, & Livneh Z. (2021). TENT4A Non-Canonical Poly(A) Polymerase Regulates DNA-Damage Tolerance via Multiple Pathways That Are Mutated in Endometrial Cancer. International Journal of Molecular Sciences, 22(13), 6957.

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Antibody characterization for DNA repair

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercially available antibodies used were as follows: rabbit anti-RAD18 (Cell Signaling, 9040S, dilution 1:2000); rabbit anti-ubiquityl-PCNA (Lys164) (Cell Signaling, 13439S, dilution 1:2000); rabbit anti-GAPDH (Cell Signaling, 5174S, dilution 1:10000); mouse anti-GAPDH (Milipore, MAB374, dilution 1:10000); rabbit anti-POLH (Cell Signaling, 13848S, dilution 1:1000); rabbit anti-CYLD (Cell Signaling, 8462S, dilution 1:1000); mouse anti-PCNA (PC-10) (Santa Cruz, sc-56, dilution 1:1000); mouse anti-FLAG M2 (Sigma-Aldrich, F1804, dilution 1:1000); rabbit anti-PTIP (PAXIP1) (Bethyl, A300-370A, dilution 1:2000); rabbit anti-preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
DTL (Bethyl, A300-948A, dilution 1:1000); rabbit anti-TRF4 (H-172) (TENT4A) (Santa Cruz, sc-98490, dilution 1:500); anti-MBP monoclonal antibody, HRP conjugated (New England Biolabs, E8038S, dilution 1:10000); IRDye 680RD goat anti-mouse IgG (LI-COR, 926-68070, dilution 1:10000); IRDye 800CW goat anti-mouse IgG (LI-COR, 926-32210, dilution 1:10000 ); IRDye 680RD goat anti-rabbit IgG (LI-COR, 926-68071, dilution 1:10000); IRDye 800CW goat anti-rabbit IgG (LI-COR, 926-32211, dilution 1:10000).

Swain U., Sehrawat U., Sarusi-Portuguez A., Friedlander G., Rotkopf R., Ebert C., Paz-Elizur T., Dikstein R., Carell T., Geacintov N.E., & Livneh Z. (2020). TENT4A poly(A) polymerase regulates translesion DNA synthesis and is mutated in endometrial cancer.

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