Blood samples were collected after an overnight fast at each timepoint, and EDTA blood samples were collected for DNA isolation and stored at −20 °C. FPG was measured using the hexokinase method, HDL and LDL cholesterol were measured using enzymatic assays, and FPI was measured using chemiluminescence assay according to standard laboratory procedures. C-reactive protein (CRP) was measured using an Image Automatic Immunoassay System (Beckman Coulter). All measurements were performed according to routine laboratory procedures. HOMA-IR was calculated as previously described [29 (link)]. Lipopolysaccharide binding protein (LBP) was measured using the HK315 HUMAN LBP ELISA Kit (Hycult Biotech, Uden, Netherlands) according to the manufacturer’s recommendations. Samples and follow-up availability dictated the sample size used for analyses at each step as illustrated in Additional file 1 : Fig. S1.
Hk315 human lbp elisa kit
Manufactured by Hycult Biotech
Sourced in Netherlands
The HK315 HUMAN LBP ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of human lipopolysaccharide-binding protein (LBP) in serum, plasma, and cell culture supernatants. The kit utilizes specific antibodies coated on a microplate to capture LBP, which is then detected using a conjugated detection antibody and a colorimetric substrate.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hk315 human lbp elisa kit
1
Biomarker Measurement Protocol for Metabolic Health
2
Immunomodulatory Effects of β2-1 Fructan
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methods used in measuring the impact of β2-1 fructan supplementation on cytokines, immune cell population analysis and physiological markers have been previously described (9) . Lipopolysaccharide binding protein (LBP) was measured using the HK315 Human LBP ELISA kit (Hycult Biotech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!