Tnf α 3707s

Amyloid beta (Aβ)_1–42, hydroxylamine hydrochloride, sodium hydroxide, hydrochloride, ferric (III) chloride hexahydrate, metaphosphoric acid, o-phthaldialdehyde, phosphoric acid, thiobarbituric acid (TBA), mannitol, sucrose, bovine serum albumin (BSA), HEPES sodium salt, 3,12-bis(carboxymethyl)-6,9-dioxa-3,12-diazatetradecanedioic acid (EGTA), 2′,7′-dichlorofluorescein diacetate (DCF-DA), digitonin, potassium chloride, potassium phosphate, HEPES, magnesium chloride, pyruvic acid, malic acid, JC-1, protease inhibitor, polyvinylidene difluoride (PVDF) membrane, sodium azide and all other chemicals were purchased from Millipore (Billerica, MA, USA). Primary antibodies such as phosphorylated c-Jun N terminal kinase (p-JNK) (sc-6254), glycogen synthase kinase 3 beta (GSK3β) (sc-9166), phosphorylated-tau (p-tau) (sc-12952), cytochrome C (sc-13560), caspase 3 (sc-56053) and β-actin (sc-69879) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other primary antibodies such as tumor necrosis factor-alpha (TNF-α) (3707S), phosphorylated-nuclear factor kappa-light-chain-enhanced of activated B cells (p-NF-κB) (#3031), phosphorylated-protein kinase (p-Akt) (#9271), BAX (#2772) and secondary antibodies such as anti-rabbit (7074S) and anti-mouse (7076S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

AUY-922 (101756-820), anti-mouse IgG HRP-linked antibody (95017-554), anti-rabbit IgG HRP-linked whole (95017-556), nitrocellulose membranes (10063-173), RIPA buffer (AAJ63306-AP) and EZBlock^TM block protease inhibitor cocktail (10190-060) were obtained from VWR (Radnor, PA, USA). APE1/Ref1 (4128S), phospho-p44/42 MAPK (Erk1/2) (9101S), p44/42 MAPK (Erk1/2) (9102S), phospho-JAK2 (3776S), JAK2 (3230S), phospho-STAT3 (9145S), STAT3 (4904S), phospho-ATR (2853S), ATR (2790S), phospho-ATM(13050S), ATM (2873S), phospho-AMPK (2535S), AMPK (2793S), IL1-β (12703S), IL-6 (12153S), IRE1α (3294S), phospho-P38 (9211S), P38 (9212S), MCP-1 (2027S) and TNFα (3707S) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Lipopolysaccharides (LPS) (L4130) and β-actin antibodies (A5441) were purchased from Sigma-Aldrich (St Louis, MO, USA). The phospho-IRE1α (PA1-16927) antibody was purchased from Thermo Scientific (Rockford, IL, USA).