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Ab15766

Manufactured by GeneTex
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AB15766 is a laboratory equipment product. It is a multi-functional device designed for various scientific applications in a laboratory setting. The core function of this product is to perform a specific task or procedure required in research and analysis activities.

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Lab products found in correlation

D9564, by Merck Group (2 mentions) Prb 278p, by Merck Group (2 mentions) Prb 435p, by Merck Group (2 mentions) Z0334, by Novus Biologicals (2 mentions) Adi spa 223, by GeneTex (2 mentions) Gtx62440, by Merck Group (2 mentions) Gtx11268, by Fortrea (2 mentions) Mab377, by Fortrea (2 mentions)

2 protocols using ab15766

1

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
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2

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, followed by washes in PBS at room temperature (RT). Cells were then blocked with 3% donkey serum in PBS containing 0.01% Triton X-100 for 1 hr at RT, incubated with primary antibodies overnight at 4°C, and then washed with PBS and incubated with secondary antibodies for 1 hr at RT. We used primary antibodies for GFAP (1:2,000; DAKO; Z0334), S100β (1:200; NOVUS; NB110–57478), ALDH1L1 (1:200; NeuroMab; 75–140), synapsin (1:1,000; SYSY; 106103), αB-crystallin (1:200; Enzo; ADI-SPA-223), MAP2 (1:500; GeneTex; GTX11268), Tuj1 (1:6,000; Covance; PRB-435P), NeuN (1:400; Millipore; MAB377), Pax6 (1:500; Covance; PRB-278P), Sox1 (1:500; Millipore; AB15766), Oligo2 (1:200; GeneTex; GTX62440), NG2 (1:500; Millipore; MAB5384), and NKX2.2 (1:50; DSHB; 745A5). Nuclei were stained with DAPI (1:6,000; Sigma; D9564).
Tian E., Sun G., Sun G., Chao J., Ye P., Warden C., Riggs A.D, & Shi Y. (2016). Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes. Cell reports, 16(3), 781-792.
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+ Expand

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