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2 protocols using cholestanetriol

1

Analytical Characterization of Sterols and Oxysterols

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Olein, stigmasterol, β-sitosterol, campesterol, brassicasterol, cholesterol, 7α-Hydroxycholesterol, 7β-Hydroxycholesterol, 7-Ketocholesterol, 5α,6α-Epoxy-cholesterol, 5β,6β-Epoxycholesterol, cholestanetriol, 19-Hydroxycholesterol, and 5α-cholestane were purchased from Sigma–Aldrich. The derivatization reagents N-methyl-N-(trimethylsilyl) heptafluorobutyramide and 1-methyl imidazole were ordered from Sigma–Aldrich. Acetone, diethyl ether, dichloromethane, hexane, and methanol were obtained from Merck & Co, Inc. FeSO4·7H2O, CuSO4·5H2O, ZnSO4, MgSO4, Na2SO4, and MnSO4 were analytically pure.
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2

Cholesterol Oxidation Products Analysis

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Cholesterol, COPs standards [7-ketocholesterol (7-keto), 6-ketocholesterol (6-keto), 7α-hydroxy-cholesterol (7α-OH), 7β-hydroxycholesterol (7β-OH), 5,6α-epoxycholesterol (5,6α-EP), 5,6β-epoxycholesterol (5,6β-EP), 25-hydroxycholesterol (25-OH), 20-hydroxycholesterol (20-OH), and cholestanetriol (triol)], butylated hydroxytoluene (BHT), pyridine, and silicic acid (100 mesh) were purchased from Sigma-Aldrich Co., LLC (Korea). Bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) + 1% trimethylchlorosilane (TMCS) was obtained from Supelco (Bellefonte, USA). HPLC grade hexane, ethyl acetate, acetone, methanol, and chloroform, Celite 545, and calcium phosphate (CaHPO4.2H2O) were purchased from Fisher Scientific Co. (USA).
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