Pxr004

Manufactured by Addgene

Sourced in United States

The PXR004 is a laboratory equipment that functions as an incubator. It is designed to provide a controlled environment for cell culture and other biological experiments.

Automatically generated - may contain errors

Lab products found in correlation

Pirfp670 n1, by Addgene (1 mentions) Pxr003, by Addgene (1 mentions) Pxr001, by Addgene (1 mentions) Pklv2 u6grna5 bbsi pgkpuro2abfp w, by Addgene (1 mentions) Pspax2, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions)

2 protocols using pxr004

Optimized crRNA Plasmid Insertion

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For crRNA insertion, we used primers with BbsI restriction sites and annealed the primers to form a double‐stranded DNA for insertion. We used pXR004 (Addgene #109054) and ligated the insert into BbsI‐restricted pXR004. We used the hU6 forward primer in the vector and the specific crRNA reverse primer for colony PCR to verify successful vector construction (Figure 1A,B).

Liu Z., Gao X., Kan C., Li L., Zhang Y., Gao Y., Zhang S., Zhou L., Zhao H., Li M., Zhang Z, & Sun Y. (2023). CRISPR‐Cas13d effectively targets SARS‐CoV‐2 variants, including Delta and Omicron, and inhibits viral infection. MedComm, 4(1), e208.

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Lentiviral Vector Construction Toolkit

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The CasRx , (pXR001; Addgene #109049), dCasRx (PXR002; Addgene #109050) plasmids, gRNA expression vectors (pXR003, pXR004; Addgene #109053 and #109054), Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2; Addgene # 12260), iRFP670 fluorescent reporter vector (piRFP670-N1; Addgene#79987) and pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene #67974) were obtained from the Addgene repository (Watertown, MA, USA).

Nguyen H., Wilson H., Jayakumar S., Kulkarni V., & Kulkarni S.R. (2021). Efficient inhibition of HIV using CRISPR/Cas13d nuclease system.

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