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Anti alpha tubulin monoclonal horseradish peroxidase conjugate

Manufactured by Cell Signaling Technology

The Anti-alpha tubulin monoclonal horseradish peroxidase conjugate is a laboratory reagent used for the detection of alpha-tubulin in biological samples. This conjugate combines a monoclonal antibody specific for alpha-tubulin with the enzyme horseradish peroxidase, which can be used to visualize the target protein through enzymatic color development.

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2 protocols using anti alpha tubulin monoclonal horseradish peroxidase conjugate

1

Immunoblotting Analysis of Signaling Proteins

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Antibodies used were anti-phospho-AKT(Ser 473), anti-phospho-AKT(Thr308), and anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb (Cell Signaling Technology, Danvers, MA), anti-Akt (pan) Rabbit mAb, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb(Cell Signaling Technology, Danvers, MA), anti-PARP rabbit mAb and Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Cell Signaling Technology, Danvers, MA), anti-alpha tubulin monoclonal horseradish peroxidase conjugate (Cell Signaling Technology, Danvers, MA), Secondary antibodies (horseradish peroxidase-linked anti-rabbit IgG and anti-mouse IgG antibodies) were obtained from Amersham Biosciences (Uppsala, Sweden).
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2

Immunoblotting with Phospho-STAT5 and Tubulin

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Antibodies used were: anti-phospho-STAT5 and anti-alpha tubulin monoclonal horseradish peroxidase conjugate (Cell Signaling Technology, Danvers, MA); anti-FLAG monoclonal antibody (Sigma-Aldrich Corp., St. Louis, MO); following recommended dilutions. Secondary antibodies (horseradish peroxidase-linked anti-rabbit IgG and anti-mouse IgG antibodies) were obtained from Amersham Biosciences (Uppsala, Sweden) and fluorescently tagged antibodies for microscopy were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) and Thermo Scientific (Waltham, MA).
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