Anti alpha tubulin monoclonal horseradish peroxidase conjugate

Manufactured by Cell Signaling Technology

The Anti-alpha tubulin monoclonal horseradish peroxidase conjugate is a laboratory reagent used for the detection of alpha-tubulin in biological samples. This conjugate combines a monoclonal antibody specific for alpha-tubulin with the enzyme horseradish peroxidase, which can be used to visualize the target protein through enzymatic color development.

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Lab products found in correlation

Horseradish peroxidase linked anti rabbit igg, by Cytiva (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Cleaved caspase 3 asp175 5a1e rabbit mab, by Cell Signaling Technology (1 mentions) Anti parp rabbit mab, by Cell Signaling Technology (1 mentions) Anti phospho stat5, by Cell Signaling Technology (1 mentions) Anti flag monoclonal antibody, by Merck Group (1 mentions)

2 protocols using anti alpha tubulin monoclonal horseradish peroxidase conjugate

Immunoblotting Analysis of Signaling Proteins

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Antibodies used were anti-phospho-AKT(Ser 473), anti-phospho-AKT(Thr308), and anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb (Cell Signaling Technology, Danvers, MA), anti-Akt (pan) Rabbit mAb, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb(Cell Signaling Technology, Danvers, MA), anti-PARP rabbit mAb and Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Cell Signaling Technology, Danvers, MA), anti-alpha tubulin monoclonal horseradish peroxidase conjugate (Cell Signaling Technology, Danvers, MA), Secondary antibodies (horseradish peroxidase-linked anti-rabbit IgG and anti-mouse IgG antibodies) were obtained from Amersham Biosciences (Uppsala, Sweden).

Majlessipour F., Zhu G., Baca N., Kumbaji M., Hwa V, & Danielpour M. (2024). Skeletal overgrowth in a pre-pubescent child treated with pan-FGFR inhibitor. Heliyon, 10(11).

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Immunoblotting with Phospho-STAT5 and Tubulin

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Antibodies used were: anti-phospho-STAT5 and anti-alpha tubulin monoclonal horseradish peroxidase conjugate (Cell Signaling Technology, Danvers, MA); anti-FLAG monoclonal antibody (Sigma-Aldrich Corp., St. Louis, MO); following recommended dilutions. Secondary antibodies (horseradish peroxidase-linked anti-rabbit IgG and anti-mouse IgG antibodies) were obtained from Amersham Biosciences (Uppsala, Sweden) and fluorescently tagged antibodies for microscopy were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) and Thermo Scientific (Waltham, MA).

Foley C.L., Al Remeithi S.S., Towe C.T., Dauber A., Backeljauw P.F., Tyzinski L., Kumar A.R, & Hwa V. (2020). Developmental adaptive immune defects associated with STAT5B deficiency in three young siblings. Journal of clinical immunology, 41(1), 136-146.

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