To analyze c-fos expression, coronal brain slices were incubated for 1 hr in blocking solution at room temperature (3% NGS, 0.3% Triton X-100) and then for 20 hr with anti-c-fos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany) for 24 hr. Sections were washed and incubated with anti-rabbit Alexa-488 secondary antibody (1:500, Jackson Immunoresearch, West Grove, PA) for 2 hr at room temperature. Afterwards, sections were washed, mounted on slides and immediately coverslipped with Fluoromount-G with DAPI (Thermo Fischer). Images were examined by fluorescence microscopy (Leica DM 5000 B) and c-fos positive cells were counted bilaterally from two LC coronal slices for each animal with ImageJ.
Anti c fos primary rabbit antibody
Manufactured by Synaptic Systems
Sourced in Germany
The anti-c-fos primary rabbit antibody is a laboratory reagent used to detect the c-fos protein, a transcription factor that is rapidly induced in response to various stimuli. It is a polyclonal antibody produced in rabbits and specifically recognizes the c-fos protein.
Automatically generated - may contain errors
2 protocols using anti c fos primary rabbit antibody
1
Immunohistochemical Analysis of c-fos Expression
2
Immunohistochemical Analysis of c-Fos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze c-fos expression, coronal brain slices were incubated for 1h in blocking solution at room temperature (3% NGS, 0.3% Triton X-100) and then for 20h with anti-c-fos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany) for 24 hr. Sections were washed and incubated with anti-rabbit Alexa-488 secondary antibody (1:500, Jackson Immunoresearch, West Grove, PA) for 2 h at room temperature. Afterwards, sections were washed, mounted on slides and immediately coverslipped with Fluoromount-G with DAPI (Thermo Fischer). Images were examined by fluorescence microscopy (Leica DM 5000 B) and cfos positive cells were counted bilaterally from two LC coronal slices for each animal with ImageJ
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!