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2 protocols using sodium hydroxide (naoh)

1

Bioactive Compound Extraction from Hemerocallis citrina

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Hemerocallis citrina Borani was obtained from Qingyang, Gansu, China. Standards of glucose, mannose, fucose, rhamnose, xylose, galactose, arabinose, glucuronic acid, ribose, and galacturonic acid were purchased from Shanghai Yuanye Biotechnology (Shanghai, China). Absolute ethanol was purchased from Fuyu Fine Chemical (Tianjin, China). Trifluoroacetic acid, methanol, sodium hydroxide, hydrochloric acid, carbazole, 1-phenyl-3-methyl-5-pyrazolone, 3,5-dinitrosalicylic acid, sulfuric acid, Coomash bright blue, chloroform, potassium bromide, sodium azide, sodium acetate, anthranone, and other reagents were purchased from Solarbio Biotechnology (Beijing, China). ABTS and FRAP assay kits were purchased from Suzhou Keming Biotechnology (Suzhou, China). Distilled water was used in these experiments.
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2

Vitamin B Analysis by HPLC

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Reference standards of thiamine hydrochloride (vitamin B1); riboflavin (vitamin B2), niacinamide (vitamin B3), D-pantothenic acid hemicalcium salt (vitamin B5), pyridoxal hydrochloride (PL), pyridoxal-5’-phosphate hydrate (PLP), pyridoxamine dihydrochloride (PM), pyridoxamine-5’-phosphate (PMP), pyridoxine hydrochloride (PN), were obtained from Sigma Chemicals (Sigma USA).1 All other chemicals and reagents used in this study were purchased from Solarbio (Beijing China),2 including sodium hydroxide, high-performance liquid chromatography (HPLC)-grade acetonitrile, phosphoric acid, and methanol. Ultra-pure water was supplied by a Milli-Q water purifier system from Millipore (Merk German).3
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