Cyclosporin h

Manufactured by Novartis

Sourced in Switzerland

Cyclosporin H is a laboratory product used for research purposes. It is a cyclic peptide compound that has been studied for its potential biological and biochemical properties. The core function of Cyclosporin H is to serve as a tool for scientific investigation, but a detailed, unbiased, and factual description of its specific applications or intended uses is not readily available.

Automatically generated - may contain errors

Lab products found in correlation

Isoluminol, by Merck Group (3 mentions) Tnf α, by Merck Group (3 mentions) Propionic acid, by Merck Group (2 mentions) Horseradish peroxidase hrp, by Roche (2 mentions) Dextran, by GE Healthcare (2 mentions) Ficoll paque, by GE Healthcare (2 mentions) Fura 2 am, by Thermo Fisher Scientific (2 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Ar420626, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

3 protocols using cyclosporin h

Neutrophil Activation Pathway Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

PSMα3 and PSMβ1 were purchased from EMC (Tübingen, Germany), Isoluminol and the FPR agonist formyl-Met-Leu-Phe (fMLF) were purchased from Sigma-Aldrich (St. Louis, MO, USA), Cyclosporin H (CysH; antagonist for FPR1) was kindly provided by Novartis Pharma (Basel, Switzerland). The hexapeptide WKYMVM (agonist for FPR2) was purchased from Alta Bioscience. The FPR2-specific antagonist PBP10 (gelsolin residues 160–169^{37 (link)}) as well as the F2Pal10 pepducin were purchased from CASLO Laboratory (Lyngby, Denmark). HRP was purchased from Roche Diagnostics (Bromma, Sweden). TNF-α, phorbol 12-myristate 13-acetate (PMA) were purchased from MilliporeSigma (Burlington, MA, USA).
All peptides were dissolved in DMSO, and subsequent dilutions of peptides and other reagents were made in Krebs–Ringer phosphate buffer (KRG; 10 mM glucose, 1.5 mM Mg²⁺, and 1 mM Ca²⁺ in dH₂O, pH 7.3).

Hu Z., Kopparapu P.K., Ebner P., Mohammad M., Lind S., Jarneborn A., Dahlgren C., Schultz M., Deshmukh M., Pullerits R., Nega M., Nguyen M.T., Fei Y., Forsman H., Götz F, & Jin T. (2022). Phenol-soluble modulin α and β display divergent roles in mice with staphylococcal septic arthritis. Communications Biology, 5, 910.

+ Open protocol

+ Expand

Peptide and Compound Synthesis for Immune Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The peptides fMLF and WKYMVM were synthesized and HPLC-purified by TAG Copenhagen A/S (Copenhagen, Denmark). Isoluminol, TNF-a, the FFA3R agonist AR420626, PMA (phorbol 12-myristate 13-acetate), propionic acid, and pertussis toxin (PTX) were purchased from Sigma (Sigma Chemical Co., St. Louis, MO, USA). Cyclosporin H was a kind gift provided by Novartis Pharma (Basel, Switzerland). Dextran and Ficoll-Paque were obtained from GE-Healthcare Bio-Science (Uppsala, Sweden). Fura 2-AM was from Molecular Probes/Thermo Fisher Scientific (Waltham, MA, USA), and horseradish peroxidase (HRP) was obtained from Boehringer Mannheim (Mannheim, Germany). The phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2yl)butanamide (PA;Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica,USA;

Mårtensson J., Holdfeldt A., Sundqvist M., Gabl M., Kenakin T.P., Björkman L., Forsman H, & Dahlgren C. (2018). Neutrophil priming that turns natural FFA2R agonists into potent activators of the superoxide generating NADPH-oxidase. Journal of leukocyte biology, 104(6).

+ Open protocol

+ Expand

Immune Cell Signaling Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isoluminol, TNF-a, ATP, propionic acid, bovine serum albumin (BSA), and fMLF were purchased from Sigma (Sigma Chemical Co., St. Louis, MO, USA). Cyclosporin H was a kind gift provided by Novartis Pharma (Basel, Switzerland). Dextran and Ficoll-Paque were obtained from GE-Healthcare Bio-Science (Uppsala, Sweden). Fura 2-AM was from Molecular Probes/Thermo Fisher Scientific (Waltham, MA, USA), and horseradish peroxidase (HRP) was obtained from Boehringer Mannheim (Mannheim, Germany). The allosteric FFAR2 modulator AZ1729 [29] were obtained from Tocris (Abingdon, UK). Subsequent dilutions of receptor ligand and other reagents were made in Krebs-Ringer Glucose phosphate buffer (KRG; 120 mM NaCl, 4.9 mM KCl, 1.7 mM KH2PO4, 8.3 mM NaH2PO4, 1.2 mM MgSO4, 10 mM glucose, and 1 mM CaCl2 in dH2O, pH 7.3).

Lind S., Hoffmann D.O., Forsman H., & Dahlgren C. (2021). Allosteric receptor modulation uncovers an FFAR2 antagonist as a positive orthosteric modulator/agonist in disguise.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!