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3 protocols using phycoerythrin pe anti human cd44

1

Isolation and Characterization of Cancer Stem Cells

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The RPMI 1640 and DMEM/F12 were bought from Gibco (Grand Island, NY, USA) and the bFGF and EGF were purchased from PEPROTECH (Rocky Hill, NJ, USA). Anti-human CD44-biotin and anti-human epithelial cell adhesion molecule (EpCAM) for magnetic-activated cell sorting (MACS), were both purchased from eBioscience (San Diego, CA, USA). Phycoerythrin (PE)-anti-human CD44 and uorescein isothiocyanate (FITC)-anti-EpCAM (eBioscience) were used for ow cytometric analysis. Mouse anti-Nanog and anti-GAPDH antibodies (Biolegend, San Diego, CA, USA), mouse anti-MMP-9, mouse anti-MMP-2, mouse anti-Bax, mouse anti-Bcl-2, rabbit anti-cleaved caspase-3, rabbit anti-TIMP-1 were purchased from Abcam (Cambrige, UK) using for western blotting.
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2

Isolation and Characterization of Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RPMI 1640 and DMEM/F12 were bought from Gibco (Grand Island, NY, USA) and the bFGF and EGF were purchased from PEPROTECH (Rocky Hill, NJ, USA). Anti-human CD44-biotin and anti-human epithelial cell adhesion molecule (EpCAM) for magnetic-activated cell sorting (MACS), were both purchased from eBioscience (San Diego, CA, USA). Phycoerythrin (PE)-anti-human CD44 and uorescein isothiocyanate (FITC)-anti-EpCAM (eBioscience) were used for ow cytometric analysis. Mouse anti-Nanog and anti-GAPDH antibodies (Biolegend, San Diego, CA, USA), mouse anti-MMP-9, mouse anti-MMP-2, mouse anti-Bax, mouse anti-Bcl-2, rabbit anti-cleaved caspase-3, rabbit anti-TIMP-1 were purchased from Abcam (Cambrige, UK) using for western blotting.
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3

Isolation and Characterization of Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RPMI 1640 and DMEM/F12 were bought from Gibco (Grand Island, NY, USA) and the bFGF and EGF were purchased from PEPROTECH (Rocky Hill, NJ, USA). Anti-human CD44-biotin and anti-human epithelial cell adhesion molecule (EpCAM) for magnetic-activated cell sorting (MACS), were both purchased from eBioscience (San Diego, CA, USA). Phycoerythrin (PE)-anti-human CD44 and uorescein isothiocyanate (FITC)-anti-EpCAM (eBioscience) were used for ow cytometric analysis. Mouse anti-Nanog and anti-GAPDH antibodies (Biolegend, San Diego, CA, USA), mouse anti-MMP-9, mouse anti-MMP-2, mouse anti-Bax, mouse anti-Bcl-2, rabbit anti-cleaved caspase-3, rabbit anti-TIMP-1 were purchased from Abcam (Cambrige, UK) using for western blotting.
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