Primary antibodies used for immunoblotting were anti-human topoisomerase IIα (mbl) (1:5000), anti-human topoisomerase IIβ (BD) (1:2000), anti-GFP (Roche) (1:2000), anti-HSP70 (Santa Cruz) (1:4000), anti-myc (abcam) (1:2000) and anti-α-tubulin (abcam) (1:10 000). Secondary antibodies were IRDye 800CW goat anti-mouse IgG (H+L) (LI-COR) (1:7000) and poly-HRP goat anti-mouse (Thermo Scientific) (1:15 000). For indirect immunofluorescence, antibodies used were anti-human topoisomerase IIα (mbl) and anti-human topoisomerase IIβ (BD) (both at 1:500). Secondary antibody used was rabbit anti-mouse FITC (Dako) (1:200).
Farr C.J., Antoniou-Kourounioti M., Mimmack M.L., Volkov A, & Porter A.C. (2014). The α isoform of topoisomerase II is required for hypercompaction of mitotic chromosomes in human cells. Nucleic Acids Research, 42(7), 4414-4426.
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