The chemicals used were
obtained from the following sources: dinitrophenyl-conjugated human
serum albumin (DNP-HSA; A6661), mouse monoclonal anti-DNP IgE (D8406),
fetal bovine serum (FBS), bovine serum albumin (BSA), and p-nitrophenyl-2-acetoamido-2-deoxy-β-d -glucopyranoside
(pNPG) from Sigma-Aldrich Japan (Tokyo, Japan). Ringer buffer was
composed of 126 mM NaCl, 4 mM KCl, 3 mM CaCl2, 1 mM MgCl2, 10 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES), and 15 mM glucose.
MT buffer was composed of 137 mM NaCl, 2.7 mM KCl, 1.8 mM CaCl2, 1 mM MgCl2, 5.6 mM glucose, 20 mM HEPES, and
0.1% (v/v) BSA. The pH of each buffer was adjusted by adding 1 M NaOH
and measured using a conventional pH meter (HORIBA).
obtained from the following sources: dinitrophenyl-conjugated human
serum albumin (DNP-HSA; A6661), mouse monoclonal anti-DNP IgE (D8406),
fetal bovine serum (FBS), bovine serum albumin (BSA), and p-nitrophenyl-2-acetoamido-2-deoxy-β-
(pNPG) from Sigma-Aldrich Japan (Tokyo, Japan). Ringer buffer was
composed of 126 mM NaCl, 4 mM KCl, 3 mM CaCl2, 1 mM MgCl2, 10 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES), and 15 mM glucose.
MT buffer was composed of 137 mM NaCl, 2.7 mM KCl, 1.8 mM CaCl2, 1 mM MgCl2, 5.6 mM glucose, 20 mM HEPES, and
0.1% (v/v) BSA. The pH of each buffer was adjusted by adding 1 M NaOH
and measured using a conventional pH meter (HORIBA).