Goat gsdmd n

Twenty-four hours after the last nasal administration in each group, 3 mice were randomly anaesthetized and killed, and the spleen, nasal bone and draining lymph nodes of the nose were collected. The nasal draining lymph nodes were collected according to the method described by Miyaga N et al. [17 (link)]. The tissues were fixed in paraformaldehyde. After 24 h, the draining lymph nodes of the nose were embedded with paraffin wax and prepared into sections. After 24 h, the nasal bone was placed into 10% EDTA decalcification solution for 2 weeks, and the decalcification solution was changed every 7 days. After 2 weeks, the nasal samples and spleen were embedded in paraffin wax and prepared into sections. Murine nasal and draining lymph node tissue samples were incubated with goat GSDMD-N (Affinity, China, DF13758) and CD11c (Servicebio, China, GB11059) antibodies. Murine splenic tissue samples were incubated with goat GSDMD-N (Affinity, China, AF4013), CD11c (Servicebio, China, GB11059), CD117 (Bioss, China, bs-2o717R), and ECP (Proteintech, China, 55338-1-AP) antibodies. Subsequently, the samples were incubated with donkey anti-goat IgG and Hoechst 33342 and then observed and imaged by confocal microscopy. The data were derived from three independent experiments.