Pierce mitochondria isolation kit

Manufactured by Thermo Fisher Scientific

The Pierce Mitochondria Isolation Kit is a laboratory product designed to isolate and purify mitochondria from various cell and tissue types. The kit provides a standardized protocol and reagents to facilitate the extraction and separation of functional mitochondria from samples.

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Lab products found in correlation

Coomassie protein assay kit, by Thermo Fisher Scientific (3 mentions) Ab6002, by Abcam (3 mentions) Sc 30080, by Santa Cruz Biotechnology (2 mentions) 3 nitrotyrosine elisa kit, by Abcam (2 mentions) Ne per nuclear and cytoplasmic extraction reagents kit, by Thermo Fisher Scientific (2 mentions) Ab37438, by Abcam (2 mentions) Ab20669, by Abcam (2 mentions) Ab8898, by Abcam (2 mentions) Ab1220, by Abcam (2 mentions) Ab2893, by Abcam (2 mentions) Dual luciferase assay system, by Promega (1 mentions) 17β estradiol e2 e2758, by Merck Group (1 mentions) Ab3576, by Abcam (1 mentions) Ab9051, by Abcam (1 mentions) Ab17339, by Abcam (1 mentions) Ab24684, by Abcam (1 mentions) Ab9053, by Abcam (1 mentions)

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Mitochondria isolation kit (295 citations)

3 protocols using pierce mitochondria isolation kit

Antibody-based Protein Quantification in Cellular Studies

Cited 1 time

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Antibodies for β-actin (sc-47778), COX2 (sc-19999), MMP1 (sc-21731), NRF1 (sc-101102), and SOD2 (sc-30080) were obtained from Santa Cruz Biotechnology. Antibodies for acetyl-histone H4 K5, K8, K12, and K16 (H4K5,8,12,16ac, #PA5-40084) were obtained from Invitrogen. Antibodies for anti-histone H3 acetyl K9, K14, K18, K23, K27 (H3K9, 14, 18, 23, 27ac, ab47915), ERα (ab3575), ERβ (ab3576), H4K20me1 (ab9051), H4K20me3 (ab9053), H4R3me1 (ab17339), H3K9me2 (ab1220), H3K9me3 (ab8898), H3K27me2 (ab24684) and H3K27me3 (ab6002), H2AX (ab20669), and γH2AX (ab2893) were obtained from Abcam. The antibody for 8-oxo-dG (4354-MC-050) was obtained from Novus Biologicals. The 3-nitrotyrosine (3-NT) was measured using 3-Nitrotyrosine ELISA Kit (ab116691 from Abcam) per manufacturers’ instructions. The mitochondrial fraction was isolated using a Pierce Mitochondria Isolation Kit (Pierce Biotechnology) according to manufacturers’ instructions. The protein concentration was measured using the Coomassie Protein Assay Kit (Pierce Biotechnology) per manufacturers’ instructions. Luciferase activity assay was carried out using the Dual-Luciferase™ Assay System (Promega) and the transfection efficiency was normalized using a cotransfected renilla plasmid (23 (link)). 17β-estradiol (E2, #E2758) and TNFα (#T0157) were obtained from Sigma.

Xiang D., Zhao M., Cai X., Wang Y., Zhang L., Yao H., Liu M., Yang H., Xu M., Li H., Peng H., Wang M., Liang X., Li L, & Yao P. (2020). Betulinic Acid Inhibits Endometriosis Through Suppression of Estrogen Receptor β Signaling Pathway. Frontiers in Endocrinology, 11, 604648.

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Quantifying Protein Modifications in Cells

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The antibodies for ERRα (ab37438), H2AX (ab20669) and γH2AX (ab2893), H3K9me2 (ab1220), and H3K9me3 (ab8898) and H3K27me3 (ab6002) were obtained from Abcam. Antibodies for β-actin (sc-47,778), ERβ (sc-137,381), and SOD2 (sc-30,080) were obtained from Santa Cruz Biotechnology. LNG and EE were obtained from Sigma. 3-Nitrotyrosine (3-NT) was measured using the 3-Nitrotyrosine ELISA Kit (ab116691 from Abcam) per manufacturers’ instructions. The mitochondrial fraction was isolated using a Pierce Mitochondria Isolation Kit (Pierce Biotechnology) per manufacturers’ instructions. Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit (Pierce Biotechnology). Protein concentration was measured using the Coomassie Protein Assay Kit (Pierce Biotechnology).

Zou Y., Lu Q., Zheng D., Chu Z., Liu Z., Chen H., Ruan Q., Ge X., Zhang Z., Wang X., Lou W., Huang Y., Wang Y., Huang X., Liu Z., Xie W., Zhou Y, & Yao P. (2017). Prenatal levonorgestrel exposure induces autism-like behavior in offspring through ERβ suppression in the amygdala. Molecular Autism, 8, 46.

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Mitochondrial and Nuclear Protein Extraction

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The antibodies for ERRα (ab37438), H2AX (ab20669), γH2AX (ab2893), H3K9me2 (ab1220), H3K9me3 (ab8898) and H3K27me3 (ab6002) were obtained from Abcam, and all the other antibodies, including β-actin (sc-47778), eNOS (sc-654), ERβ (sc-137381) and SOD2 (sc-30080) were obtained from Santa Cruz Biotechnology. The mitochondrial fraction was isolated using a Pierce Mitochondria Isolation Kit (Pierce Biotechnology) according to manufacturers’ instructions. Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit (Pierce Biotechnology). Protein concentration was measured using the Coomassie Protein Assay Kit (Pierce Biotechnology) according to manufacturers’ instructions.

Xie W., Ren M., Li L., Zhu Y., Chu Z., Zhu Z., Ruan Q., Lou W., Zhang H., Han Z., Huang X., Xiang W., Wang T, & Yao P. (2017). Perinatal testosterone exposure potentiates vascular dysfunction by ERβ suppression in endothelial progenitor cells. PLoS ONE, 12(8), e0182945.

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