Huvec c

Twenty paired placental tissues from PE women, who were diagnosed with PE and underwent deliveries at International Peace Maternity and Child Health Hospital between 2016 and 2018, and women with normal pregnancies were collected. All tissue samples were immediately frozen in liquid nitrogen and stored at −80°C prior to RNA extraction.
Two cell lines were used in this study: the human trophoblast cell line HTR‐8/SVneo, and the HUVEC‐C cell line, which was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). HTR‐8/SVneo cells were cultured in RPMI 1640 and HUVEC‐C cells were cultured in ECM (KeyGEN, Nanjing, China), to which 10% fetal bovine serum (GIBCO, BRL, Invitrogen, Carlsbad, CA, USA),100 U/mL penicillin and 100 mg/mL streptomycin (Invitrogen) were added. Cells were incubated in a humidified atmosphere of 5% CO₂ at 37°C and were passaged every 3 days.

Human mononuclear macrophages (THP-1, No. ZQ 0086) were purchased from the Shanghai Zhongqiao Biological Research Institute (Shanghai, China). Human umbilical vein endothelial cells (HUVEC-C, Product Number: 16-150828) were purchased from Jiangsu KeyGEN BioTECH Corp., Ltd. (Jiangsu, China). Both cell lines were purchased with an evaluation certificate.

We selected three cell lines that were related to pregnancy. HTR-8/SVneo Cells was generous furnished by Prof. Charies Graham, Queen’s University, Canada. And JEG-3, HUVEC-C, WISH and BeWo cells were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). HTR/SVneo, JEG-3, BeWo, WISH and HUVEC-C cell lines were cultured in RPMI 1640, MEM, F12K, RPMI 1640 and ECM (KeyGEN, Nanjing, China), respectively, which added to 10% fetal bovine serum (GIBCO, BRL, Invitrogen, Carisbad, CA, USA),100 U/ml penicillin and 100 mg/ml streptomycin (Invitrogen) in humidified air at 37 °C/5% CO₂.