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Lentiviral mission short hairpin rna shrna clones targeting

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Lentiviral Mission short hairpin RNA (shRNA) clones are a type of lab equipment used for gene silencing studies. They consist of short hairpin RNA sequences designed to target specific genes for knockdown. The core function of these clones is to enable efficient and stable gene silencing through lentiviral transduction.

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Lab products found in correlation

Sw28 centrifuge rotor, by Beckman Coulter (2 mentions) Pspax2 packaging plasmid, by Addgene (2 mentions)

Close spelling variants of this product

MISSION shRNA clones Lentiviral shRNA clones Short hairpin RNA ( MISSION clone ShRNA clones MISSION shRNA ShRNA targeting Lentiviral shRNAs MISSION RNA

2 protocols using lentiviral mission short hairpin rna shrna clones targeting

1

Lentiviral shRNA Knockdown of UBE4B

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Three lentiviral Mission short hairpin RNA (shRNA) clones targeting UBE4B were obtained from Millipore-Sigma. HEK293T cells were transfected with the lentiviral shRNA-targeting vectors, psPAX2 packaging plasmid (Addgene) and vesicular stomatitis virus glycoprotein (VSV-G). After 48 h, the supernatants were collected and centrifuged at 25,000 rpm using a Beckman SW28 centrifuge rotor for 2 h at 4°C. The supernatants were removed, and the pellets were re-suspended in ice-cold phosphate-buffered saline (PBS). Viral stocks were used to infect cell lines.
Mohanty S., Han T., Choi Y.B., Lavorgna A., Zhang J, & Harhaj E.W. (2020). The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation. PLoS Pathogens, 16(12), e1008504.
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2

Lentiviral shRNA Knockdown of UBE4B

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three lentiviral Mission short hairpin RNA (shRNA) clones targeting UBE4B were obtained from Millipore-Sigma. HEK293T cells were transfected with the lentiviral shRNA-targeting vectors, psPAX2 packaging plasmid (Addgene) and vesicular stomatitis virus glycoprotein (VSV-G). After 48 h, the supernatants were collected and centrifuged at 25,000 rpm using a Beckman SW28 centrifuge rotor for 2 h at 4°C. The supernatants were removed, and the pellets were re-suspended in ice-cold phosphate-buffered saline (PBS). Viral stocks were used to infect cell lines.
Mohanty S., Han T., Choi Y.B., Lavorgna A., Zhang J., & Harhaj E.W. (2020). The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation.
+ Open protocol
+ Expand

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