Histology and IHC were performed as described previously (Martínez et al., 2009 (link)) using the following antibodies and dilutions: mouse monoclonal anti-GFP (Roche, 1:500), rabbit polyclonal anti-CK14 (Covance, 1:1,000), rabbit polyclonal anti-CK10 (Covance, 1:300), rabbit polyclonal anti-Loricrin (Covance, 1:500), rabbit polyclonal anti-p53 (CM5 Novocastra, 1:200), mouse monoclonal anti-Ki67 (Master Diagnostica, 1:200), mouse monoclonal anti-Histone 3 phospho-Ser10 (Millipore, 1:200), mouse monoclonal anti-Histone 2 phospho-Ser139 (γH2AX, Millipore, 1:100), mouse monoclonal anti-p63 (Neo Markers, 1:100), and rat monoclonal anti-BrdU (Abcam, 1:50). Images were captured in a bright-field microscope under 20× or 40× magnification and quantified using ImageJ. At least 500–1,000 nuclei per skin region and mouse were scored, and values were represented as the percentage of positive nuclei per mouse.
Rabbit polyclonal anti loricrin
Manufactured by Fortrea
Rabbit polyclonal anti-Loricrin is a laboratory reagent used for the detection and analysis of the loricrin protein. Loricrin is a structural protein found in the cornified cell envelope of mammalian epidermis. This antibody can be used in various immunodetection techniques to identify and study the expression and localization of loricrin in biological samples.
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Lab products found in correlation
Mouse monoclonal anti gfp, by Roche (2 mentions)
Rabbit polyclonal anti p53, by Leica (2 mentions)
Mouse monoclonal anti histone 3 phospho ser10, by Merck Group (2 mentions)
Mouse monoclonal anti histone 2 phospho ser139 γh2ax, by Merck Group (2 mentions)
Rat monoclonal anti brdu, by Abcam (2 mentions)
Rabbit polyclonal anti ck14, by Fortrea (2 mentions)
Rabbit polyclonal anti ck10, by Fortrea (2 mentions)
2 protocols using rabbit polyclonal anti loricrin
1
Immunohistochemical Analysis of Skin
2
Immunohistochemical Analysis of Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Histology and IHC were performed as described previously (Martínez et al., 2009 (link)) using the following antibodies and dilutions: mouse monoclonal anti-GFP (Roche, 1:500), rabbit polyclonal anti-CK14 (Covance, 1:1,000), rabbit polyclonal anti-CK10 (Covance, 1:300), rabbit polyclonal anti-Loricrin (Covance, 1:500), rabbit polyclonal anti-p53 (CM5 Novocastra, 1:200), mouse monoclonal anti-Ki67 (Master Diagnostica, 1:200), mouse monoclonal anti-Histone 3 phospho-Ser10 (Millipore, 1:200), mouse monoclonal anti-Histone 2 phospho-Ser139 (γH2AX, Millipore, 1:100), mouse monoclonal anti-p63 (Neo Markers, 1:100), and rat monoclonal anti-BrdU (Abcam, 1:50). Images were captured in a bright-field microscope under 20× or 40× magnification and quantified using ImageJ. At least 500–1,000 nuclei per skin region and mouse were scored, and values were represented as the percentage of positive nuclei per mouse.
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