Ezna genomic dna isolation kit

A total of 40 Cronobacter isolates recovered from spices and cereal food samples in China between September 2014 and June 2015 were analyzed (Table 1). Twenty-one strains were from spices and 19 from cereals. These strains have been confirmed as Cronobacter spp. by genus specific PCR confirmation based on the outer membrane protein A (OmpA) and internal transcribed spacer (ITS) gene, and 16S rRNA sequencing in our previous work (Li Y. H. et al., 2016 (link); Li et al., 2017 (link)). The bacterial strains were routinely grown in Tryptic Soy Broth (TSB; QingDao Hope Bio-technology Co., Ltd, Qingdao, China) at 37°C overnight without shaking. Then genomic DNA was extracted with the EZNA Genomic DNA Isolation Kit (Omega Bio-Tek, Doraville, USA) according to the manufacturer's protocols.

Genomic DNA was isolated from PBMCs by using E.Z.N.A. Genomic DNA isolation kit (Omega Bio-Tek). Global 5mC and 5hmC content in the PBMCs were quantified with 5mC DNA ELISA Kit and Quest 5hmC DNA ELISA kit (ZYMO, USA). The detailed information is presented in the Supplementary Materials.

Human saliva and dental plaques samples of 10 healthy donors (five female and five male; mean age 24 ± 3 years) were obtained from Wuxi No. 2 People’s Hospital (Wuxi, Jiangsu Province, China) according to the method reported by Krajden [30 (link)]. Lactobacillus strains were isolated by plating the serial diluted samples on MRS agar and cultivated at 37 °C under microaerophilic conditions. Genomic DNA of Lactobacillus strains were extracted using EZNA genomic DNA isolation kits (Omega Bio-Tek, Doraville, USA). Strains were identified by analysis of 16S rDNA (amplified by primers 16SF 5′-TACGGYTACCTTGTTACGACTT-3′ and 16SR 5′-AGAGTTTGATCMTGGCTCAG-3′) and pheS (amplified by primers pheSF 5′-TTCCCATTTACGGAGCCTTCTG-3′ and pheSR 5′-GCACCATACCGGCACCTAACAC-3′) sequences and blasted against the NCBI database.