The 5:3:2 DOPE/DOPS/DOPC lipid
mixture (ESC, product no. 790304), porcine brainl -α-phosphatidylserine
(PS, product no. 840032), porcine brainl -α-phosphatidylcholine
(PC, product no. 840053), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l -serine (POPS, product no. 840034) were
all purchased from Avanti Polar Lipids (Alabaster, AL). ESC, a 7:3
(w:w) porcine brain PC/PS mixture, and synthetic POPS lipid molecules
were dissolved in chloroform by vortexing and dried under a N2 stream, followed by exposure to vacuum for 1 h. Lipid films
were resuspended in PBS buffer by vortexing to a final lipid concentration
of 30 mM. Small unilamellar vesicles (SUVs) were then generated by
sonicating the resuspended lipids in a bath sonicator (P30H, Elma,
Singen, Germany) at 37 kHz and 50% power for ∼2 h at room temperature
to reach optical transparency.
mixture (ESC, product no. 790304), porcine brain
(PS, product no. 840032), porcine brain
(PC, product no. 840053), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-
all purchased from Avanti Polar Lipids (Alabaster, AL). ESC, a 7:3
(w:w) porcine brain PC/PS mixture, and synthetic POPS lipid molecules
were dissolved in chloroform by vortexing and dried under a N2 stream, followed by exposure to vacuum for 1 h. Lipid films
were resuspended in PBS buffer by vortexing to a final lipid concentration
of 30 mM. Small unilamellar vesicles (SUVs) were then generated by
sonicating the resuspended lipids in a bath sonicator (P30H, Elma,
Singen, Germany) at 37 kHz and 50% power for ∼2 h at room temperature
to reach optical transparency.