Spca 1

The human LUAC cell lines A549 and H1299 were purchased from American Type Culture Collection (ATCC) and maintained in RPMI‐1640 (KeyGen, Nanjing, China), except for SPCA‐1, which was maintained in DMEM (KeyGen, Nanjing, China), and both media were supplemented with 10% FBS (Life Technologies) at 37°C in a humidified atmosphere with 5% CO2. The FAK inhibitor PF-562271 (ab141360) was purchased from Abcam (Cambridge, UK). Cells were authenticated by STR analysis at Guangzhou Cellcook Biotech Co., Ltd. (Guangzhou, China) Characterized Cell Line Core Facility within the last three years and routinely tested negative for mycoplasma contamination.

The human LUAC cell lines HBE, A549, H1299, H1975, PC-9, and SPCA-1 were purchased from ATCC and maintained in RPMI-1640 (KeyGen), except for SPCA-1, which was maintained in DMEM (KeyGen), and both media were supplemented with 10% fetal bovine serum (FBS; Life Technologies) at 37°C in a humidified atmosphere with 5% CO₂.

Both the human lung adenocarcinoma A549 (RRID: CVCL_0023) and SPCA1 (RRID: CVCL_6955) cell lines were obtained from Jiangsu Provincial Key Laboratory of Geriatrics (Nanjing, Jiangsu, China), and erastin/RSL3-resistant lung adenocarcinoma cell lines were kindly provided by the Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University (Nanjing, Jiangsu, China). The A549 (Shanghai Biowing Biotechnology Application, Shanghai, China) and SPCA1 (KeyGEN BioTECH, Shanghai, China) cell lines were verified via STR analysis. In addition, the cells were tested for the presence of mycoplasma infection using a PCR assay. All the cell lines were cultured in DMEM (Sigma-Aldrich, Darmstadt, Germany) with 100 μg/mL streptomycin, 100 U/mL penicillin (Gibco, Billings, MT, USA), and 10% fetal bovine serum (FBS) (Invitrogen, Waltham, MA, USA) in a humidified atmosphere (Thermo Fisher Scientific, Waltham, MA, USA) that was 5% CO₂. All cells used for experiments were actively passaged for less than 3 weeks and were in the logarithmic growth phase.