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Diagnostic reagent kit

Manufactured by Pars Azmoon

The Diagnostic Reagent kit is a laboratory equipment designed for performing diagnostic tests. The kit contains a set of pre-prepared reagents that are used to analyze samples and detect the presence of specific substances or indicators. The core function of the kit is to provide the necessary tools and materials for conducting various diagnostic procedures in a laboratory setting.

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2 protocols using diagnostic reagent kit

1

Antioxidant Enzyme Activities

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One unit of SOD activity is defined as the amount of enzyme required to inhibit the reduction of NBT by 50%. The values are expressed in units/mg protein.
Glutathione peroxidase (Gpx, EC 1.11.1.9) activity was measured by a GPx Diagnostic kit (Biorex, Muckamore, UK) according to the manufacturers' protocol.
Lactate dehydrogenase (LDH, EC 1.1.1.27) and alkaline phosphatase (ALP, EC 3.1.3.1) activities were assayed by a Diagnostic Reagent kit (Pars Azmoon, Tehran, Iran) according to the manufacturer's protocol.
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2

Cardiac Oxidative Stress Assessment

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Cardiac levels of oxidative stress indices including SOD, GPx, and TAC were measured by biochemical kits, following the manufacturer's instruction (Randox Company, England). TAC was determined by Randox's total antioxidant status kit, as described by Miller et al. [38 (link)]. Cardiac SOD was measured, according to Breinholt et al., using a RANSOD kit (Randox Labs Crumlin, UK) [39 (link)]. Also, cardiac GPx was assayed by a RANSEL Kit (Randox Labs Crumlin, UK), as described by Paglia and Valentine [40 (link)]. Thiobarbituric acid was used to measure the MDA level; TBARS were determined by Esterbauer and Cheeseman [41 (link)]. In addition, serum glucose was evaluated spectrophotometrically using a diagnostic reagent kit (Pars Azmoon Company, Tehran, Iran). Furthermore, serum concentration of insulin was determined by the ELISA method (Shanghai Crystal Day Biotech Co., Ltd., China).
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