Cardiac levels of oxidative stress indices including SOD, GPx, and TAC were measured by biochemical kits, following the manufacturer's instruction (Randox Company, England). TAC was determined by Randox's
total antioxidant status kit, as described by Miller et al. [38 (
link)]. Cardiac SOD was measured, according to Breinholt et al., using a
RANSOD kit (Randox Labs Crumlin, UK) [39 (
link)]. Also, cardiac GPx was assayed by a
RANSEL Kit (Randox Labs Crumlin, UK), as described by Paglia and Valentine [40 (
link)]. Thiobarbituric acid was used to measure the MDA level; TBARS were determined by Esterbauer and Cheeseman [41 (
link)]. In addition, serum glucose was evaluated spectrophotometrically using a
diagnostic reagent kit (Pars Azmoon Company, Tehran, Iran). Furthermore, serum concentration of insulin was determined by the ELISA method (Shanghai Crystal Day Biotech Co., Ltd., China).
Sefidgari-Abrasi S., Karimi P., Roshangar L., Morshedi M., Bavafa-Valenlia K., Saghafi-Asl M., Mohiti S, & Rahimiyan-Heravan M. (2020). Lactobacillus plantarum And Inulin: Therapeutic Agents to Enhance Cardiac Ob Receptor Expression and Suppress Cardiac Apoptosis in Type 2 Diabetic Rats. Journal of Diabetes Research, 2020, 4745389.