Influenza virus and S. pneumoniae were cultured as described previously [14 (link)]. Influenza virus A/Puerto Rico/8/34 H1N1 strain (PR8) was obtained from the American Type Culture Collection (ATCC), propagated in embryonated eggs, and the viral titers were determined by plaque assay. S. pneumoniae serotypes 3 (A66.1 Xen 10), 4 (TIGR), and 19F (clinical isolate from Singapore), were cultured till mid-logarithmic phase in brain-heart infusion broth (Sigma) supplemented with 5% heat-inactivated fetal bovine serum (FBS) under anaerobic conditions. Type 4cps4D-, a capsule mutant of serotype 4 that lacks the cps4D gene (Supplementary Figure 1B ) was generated previously [35 (link)], and was cultured under the same conditions as its wild-type counterpart.
Influenza virus a puerto rico 8 34 h1n1 strain pr8
Manufactured by American Type Culture Collection
The Influenza virus A/Puerto Rico/8/34 H1N1 strain (PR8) is a laboratory-adapted strain of the influenza A virus subtype H1N1. It is a widely used reference strain for influenza research and vaccine development. This virus strain serves as a core tool for studying the biology, epidemiology, and pathogenesis of influenza viruses.
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Lab products found in correlation
Brain heart infusion broth, by Merck Group (3 mentions)
Cefadroxil, by Merck Group (1 mentions)
Sodium phosphate monobasic, by Merck Group (1 mentions)
Formalin, by Merck Group (1 mentions)
Sodium phosphate dibasic, by Merck Group (1 mentions)
Amoxicillin, by Merck Group (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
3 protocols using influenza virus a puerto rico 8 34 h1n1 strain pr8
1
Influenza and Streptococcus pneumoniae Co-Infection Protocol
2
Propagation and Serotyping of Influenza and Pneumococcus
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Influenza virus A/Puerto Rico/8/34 H1N1 strain (PR8) was obtained from the American Type Culture Collection and propagated in embryonated eggs. Viral titers were determined by plaque assay. Serotypes 3 and 19F of S. pneumoniae were derived from clinical isolates in Singapore and cultured in brain heart infusion broth (Sigma 53286) supplemented with 5% heat-inactivated fetal bovine serum (FBS) under anaerobic conditions until the mid-logarithmic phase.
3
Influenza and Pneumococcus Experimental Protocols
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Metolose (90SH-100000SR) was acquired from
Shin-Etsu Chemical. Amoxicillin, cefadroxil, monobasic sodium phosphate,
dibasic sodium phosphate, methanol, acetonitrile, paraformaldehyde,
formalin, hematoxylin, and eosin were purchased from Sigma-Aldrich.
The influenza virus A/Puerto Rico/8/34 H1N1 strain (PR8) was obtained
from the American Type Culture Collection and propagated in embryonated
eggs. Viral titers were determined by plaque assay. Serotype 19F S. pneumoniae, obtained from a clinical isolate from
Singapore and cultured in a brain–heart infusion Broth (Sigma-Aldrich),
was added with 5% heat-inactivated fetal bovine serum, under anaerobic
conditions in an incubator (Thermo Fisher) at 37 °C until the
mid-logarithmic phase.
Shin-Etsu Chemical. Amoxicillin, cefadroxil, monobasic sodium phosphate,
dibasic sodium phosphate, methanol, acetonitrile, paraformaldehyde,
formalin, hematoxylin, and eosin were purchased from Sigma-Aldrich.
The influenza virus A/Puerto Rico/8/34 H1N1 strain (PR8) was obtained
from the American Type Culture Collection and propagated in embryonated
eggs. Viral titers were determined by plaque assay. Serotype 19F S. pneumoniae, obtained from a clinical isolate from
Singapore and cultured in a brain–heart infusion Broth (Sigma-Aldrich),
was added with 5% heat-inactivated fetal bovine serum, under anaerobic
conditions in an incubator (Thermo Fisher) at 37 °C until the
mid-logarithmic phase.
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