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Rabbit polyclonal antibody to gfp

Manufactured by Thermo Fisher Scientific
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The Rabbit polyclonal antibody to GFP is a laboratory reagent used to detect and quantify the presence of green fluorescent protein (GFP) in biological samples. This antibody is produced by immunizing rabbits with purified GFP, resulting in the generation of polyclonal antibodies that recognize multiple epitopes on the GFP protein.

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Lab products found in correlation

Zombie nir fixable viability kit, by BioLegend (2 mentions) Foxp3 intracellular staining reagents, by Thermo Fisher Scientific (2 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (2 mentions) Annexin 5 apoptosis detection kit, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions)

Close spelling variants of this product

Rabbit antibody to GFP GFP polyclonal antibody Rabbit GFP antibody GFP antibody

2 protocols using rabbit polyclonal antibody to gfp

1

Multiparametric Analysis of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for CD3 (2C11), CD4 (RM4-5), CD8a (53-6.7), CD44 (IM7), CD62L (MEL-14), TCR beta chain (H57-597), CD25 (ebio3C7 or PC61), CD45.1 (A20), CD45.2 (104), Ly6G (1A8), Ly6C (HK1.4), B220 (RA3-6B2), major histocompatibility complex (MHC) class II (M5/114.15.2), CD11b (M1/70), CD11c (N418), Siglec-F (E50-2440), Foxp3 (FJK-16 s), IFNγ (XMG1.2), IL-17A (TC11-18H10.1), IL-13 (eBio13A), IL-10 (JES5-16E3), KAP1 (20A1), Blimp-1 (5E7), and CTLA4 (UC10-4B9) were from eBioscience, BioLegend, or BD Biosciences. For YFP staining, rabbit polyclonal antibody to GFP (Thermo Fisher Scientific) was used. Cells were stimulated with phorbol 12-myristate 13-acetate (PMA) (20 ng/mL), ionomycin (1 μg/mL), and monensin (2 μM) for 5 hr for cytokine staining. Intracellular staining was conducted using Foxp3 intracellular staining reagents from eBioscience. Dead cells were excluded from the analyses using the Zombie NIR Fixable Viability Kit (BioLegend). An apoptosis assay was performed using the Annexin V Apoptosis Detection Kit (eBioscience). CD4+ T cells were isolated using the CD4+ T Cell Isolation Kit (Miltenyi Biotec), and Tregs were further purified by YFP using FACSAria II (BD Biosciences).
Tanaka S., Pfleger C., Lai J.F., Roan F., Sun S.C, & Ziegler S.F. (2018). KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell reports, 23(3), 796-807.
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2

Multiparametric Analysis of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for CD3 (2C11), CD4 (RM4-5), CD8a (53-6.7), CD44 (IM7), CD62L (MEL-14), TCR beta chain (H57-597), CD25 (ebio3C7 or PC61), CD45.1 (A20), CD45.2 (104), Ly6G (1A8), Ly6C (HK1.4), B220 (RA3-6B2), major histocompatibility complex (MHC) class II (M5/114.15.2), CD11b (M1/70), CD11c (N418), Siglec-F (E50-2440), Foxp3 (FJK-16 s), IFNγ (XMG1.2), IL-17A (TC11-18H10.1), IL-13 (eBio13A), IL-10 (JES5-16E3), KAP1 (20A1), Blimp-1 (5E7), and CTLA4 (UC10-4B9) were from eBioscience, BioLegend, or BD Biosciences. For YFP staining, rabbit polyclonal antibody to GFP (Thermo Fisher Scientific) was used. Cells were stimulated with phorbol 12-myristate 13-acetate (PMA) (20 ng/mL), ionomycin (1 μg/mL), and monensin (2 μM) for 5 hr for cytokine staining. Intracellular staining was conducted using Foxp3 intracellular staining reagents from eBioscience. Dead cells were excluded from the analyses using the Zombie NIR Fixable Viability Kit (BioLegend). An apoptosis assay was performed using the Annexin V Apoptosis Detection Kit (eBioscience). CD4+ T cells were isolated using the CD4+ T Cell Isolation Kit (Miltenyi Biotec), and Tregs were further purified by YFP using FACSAria II (BD Biosciences).
Tanaka S., Pfleger C., Lai J.F., Roan F., Sun S.C, & Ziegler S.F. (2018). KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell reports, 23(3), 796-807.
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