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Hyclone high glucose dmem

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Hyclone high glucose DMEM is a cell culture medium formulated with a high concentration of glucose, which provides an energy source for the growth and maintenance of various cell types. It is a basal medium that can be customized by the addition of supplements as required for specific cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Mia paca 2, by Thermo Fisher Scientific (1 mentions) Panc 1, by Thermo Fisher Scientific (1 mentions) Hyclone mem ebss, by Thermo Fisher Scientific (1 mentions) Saos 2, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Panc 1, by American Type Culture Collection (1 mentions) Bxpc 3, by American Type Culture Collection (1 mentions) Aspc 1, by American Type Culture Collection (1 mentions) Saos 2, by American Type Culture Collection (1 mentions) Mia paca 2, by American Type Culture Collection (1 mentions) Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

HyClone® DMEM high glucose medium Hyclone Dulbecco’s modified Eagle’s medium (DMEM)/high glucose High-glucose DMEM HyClone® DMEM DMEM-glucose High glucose High DMEM HyClone

5 protocols using hyclone high glucose dmem

1

Cell Culture and Viral Vectors for Cancer Research

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The human lung fibroblast WI38 cell line, osteosarcoma cell line SAOS-2 (p53 null and truncated RB1), and the pancreatic cancer cell lines, BxPC-3, AsPC-1, MIA PaCa-2, and PANC-1 were purchased from the American Type Culture Collection (Manassas, VA, USA). WI38 cells were grown in Hyclone MEM/EBSS (ThermoFisher Scientific, Waltham, MA) media supplemented with 10% research grade fetal bovine serum (FBS) (PAA Laboratories, Dartmouth, MA) and 1% Penicillin Streptomycin (Corning, Corning, NY) and SAOS-2, MIA PaCa-2, and PANC-1 cells were grown in Hyclone High Glucose DMEM (ThermoFisher Scientific, Waltham, MA) supplemented with 10% FBS and 1% Penicillin Streptomycin. BxPC-3 and AsPC-1 were cultured in RPMI supplemented with 10% or 15% FBS (respectively) and 1% Penicillin Streptomycin. Cells were cultured at 37°C in a humidified 5% CO2 incubator.
Ad.CMV (adenovirus with CMV promoter) and Ad.CMV.p53 (Adenovirus containing wild-type p53 gene under control of CMV promoter) viral vectors were generated using the AdEasy system (Carlsbad, CA). The Ad.CMV.pRb (Adenovirus containing RB1 gene cDNA under control of CMV promoter) vector was provided by Dr. Juan Fueyo (M.D. Anderson Cancer Center, The University of Texas). The Ad.GFP and Ad.GFP.RGS16 viruses were purchased from Vector Biolabs (Philadelphia, PA). Viruses were amplified and titered as previously described [81 (link)-83 (link)].
Carper M.B., Denvir J., Boskovic G., Primerano D.A, & Claudio P.P. (2014). RGS16, a novel p53 and pRb cross-talk candidate inhibits migration and invasion of pancreatic cancer cells. Genes & Cancer, 5(11-12), 420-435.
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2

Culturing HEK293T and HT1080 cells

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HEK293T and HT1080 fibroblasts were cultured in Hyclone high glucose DMEM (Thermo Scientific SH30022.01, Waltham, MA) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals S11550, Lawrenceville, GA) and 50 U/mL penicillin and streptomycin (Lonza 17-602E, Walkersville, MD). Cell lines were maintained at 37°C and 5% CO2.
Browning D.L., Everson E.M., Leap D.J., Hocum J.D., Wang H., Stamatoyannopoulos G, & Trobridge G.D. (2016). Evidence for the in vivo safety of insulated foamy viral vectors. Gene therapy, 24(3), 187-198.
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3

Generation and Culture of Luciferase-Expressing MDA-MB-231 Cells

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The human MDA-MB-231 (ATCC Cat# CRL-12532, RRID:CVCL_0062) mammary carcinoma cell line (24 (link)) expressing firefly luciferase was a kind gift of Joan Massagué (Memorial Sloan Kettering Cancer Center, New York, NY). These cells were further retrovirally infected to express ZsGreen using MSCV-ZsGreen-2A-Puro (25 (link)). Cells were cultured in HyClone high-glucose DMEM (ThermoFisher, San Jose, CA) supplemented with 2 mM glutamine and 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA) in a 37°C incubator with 5% CO2. Production of retrovirus and lentivirus, as well as transduction of cells, was performed as previously described (26 (link)). Cells were tested monthly for mycoplasma using a PCR-based Universal Mycoplasma Detection Kit (ATCC, Manassas, VA). All cell lines were used for experiments 1–2 passages after thawing.
Hebert J.D., Myers S.A., Naba A., Abbruzzese G., Lamar J.M., Carr S.A, & Hynes R.O. (2020). Proteomic profiling of the ECM of xenograft breast cancer metastases in different organs reveals distinct metastatic niches. Cancer research, 80(7), 1475-1485.
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4

Culturing HEK293T and HT1080 cells

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HEK293T and HT1080 fibroblasts were cultured in Hyclone high glucose DMEM (Thermo Scientific SH30022.01, Waltham, MA) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals S11550, Lawrenceville, GA) and 50 U/mL penicillin and streptomycin (Lonza 17-602E, Walkersville, MD). Cell lines were maintained at 37°C and 5% CO2.
Browning D.L., Everson E.M., Leap D.J., Hocum J.D., Wang H., Stamatoyannopoulos G, & Trobridge G.D. (2016). Evidence for the in vivo safety of insulated foamy viral vectors. Gene therapy, 24(3), 187-198.
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5

Culturing Melanoma and Mammary Carcinoma Cell Lines

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The human melanoma MA2 cell line41 (link) and the human mammary carcinoma LM2-mCherry cell line (originally developed in the lab of Joan Massagué24 (link), a kind gift from Daniel Haber) were both cultured in HyClone high-glucose DMEM (ThermoFisher) supplemented with 2 mM glutamine and 10% fetal bovine serum (FBS, Invitrogen) in a 37 °C incubator with 5% CO2. Phase-contrast images of cells in culture were taken with a Zeiss Axiovert 200 microscope using a 10× or 5× objective.
Hebert J.D., Tian C., Lamar J.M., Rickelt S., Abbruzzese G., Liu X, & Hynes R.O. (2020). The scaffold protein IQGAP1 is crucial for extravasation and metastasis. Scientific Reports, 10, 2439.
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