PET imaging (Inveon, Siemens) was performed under 2% isoflurane anaesthesia followed by a CT scan (MicroCAT, Siemens). Optional FDG-PET (10 min static scan) was conducted 24 h prior to immunoPET imaging, using 3.7 MBq of [18F]FDG, followed by 1 hour conscious uptake. For immunoPET, mice were injected with 15 to 20 µg (3.7–7.4 MBq) of either [18F]FB-GAcDb or [18F]FBEM-GAcDb. 1 or 2 hour dynamic PET acquisitions were followed by ten minute static scans at 2, 4 and 6 h post injection (p.i.). PET images were reconstructed using maximum a posteriori (OSEM MAP) algorithm and PET and CT images are presented as maximum intensity projection (MIP) overlays. AMIDE software was used for image analysis (35 (link)). For quantitation of the PET signal in specific organs, 3D regions of interest (ROI) were drawn and the mean voxel value was converted to %ID/ccROI using the decay corrected injected dose and an empirically determined cylinder factor for 18F.
Ex vivo biodistributions were performed by weighing and gamma counting tissues and calculating %ID/g values based on standards containing 1% of the injected dose.
Ex vivo biodistributions were performed by weighing and gamma counting tissues and calculating %ID/g values based on standards containing 1% of the injected dose.