Total 50 mg of kidney from sham-or CLP-operated mice were placed in the liquid provided with the kit and homogenized on ice. The protein content was measure with Bradford method (Beyotime Biotechnology, Shanghai, China). Caspase-3 activity levels were acquired by caspase-3 assay kit (Solarbio, Beijing, China) based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA following the protocols from the manufacturer. This analysis was used to further confirm the data of immunostaining with cleaved caspase-3 antibody, and TUNEL kit.
Caspase 3 assay kit
Manufactured by Solarbio
Sourced in China
The Caspase-3 assay kit is a laboratory tool used to detect and measure the activity of the enzyme Caspase-3. Caspase-3 is a key mediator of apoptosis, a process of programmed cell death. The kit provides a quantitative colorimetric or fluorometric method for measuring Caspase-3 activity in cell lysates, tissue extracts, or purified enzyme preparations.
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Lab products found in correlation
2 protocols using caspase 3 assay kit
1
Caspase-3 Activity Assay in Kidney Tissue
2
Vascular Caspase and MMP Activity Assay
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Caspase3/9 activity in the vascular tissue or cell homogenates were evaluated using the Caspase3 Assay Kit (Solarbio, BC3830) or Caspase9 Assay Kit (Solarbio, BC3890) according to manufacturer's instructions. MMP2/9 activity in the vascular tissue were evaluated using the MMP2 Assay Kit (GMS50070.5, GENMED SCIENTIFICS INC, U.S.A) or MMP9 Assay Kit (GMS50074.5, GENMED SCIENTIFICS INC, U.S.A) according to manufacturer's instructions.
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