Fresh apple was provided by Xuanwu Fruit Store, Nanjing, Jiangsu Province. Ethanol, acetonitrile, Folin-Ciocalteu reagent, organic acid standards (oxalate acid, pyruvic acid, malic acid, citric acid, succinic acid and lactic acid), phenolic acid standards (gallic acid, protocatechuic acid, catechin, procyanidin B2, chlorogenic acid, hydroxybenzoic acid, caffeic acid, ferulic acid, rutin and quercetin), amino acid standards (Asp, Thr, Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Phe, Lys, His, Arg, Pro) and sugar standards (fructose, sorbitol, glucose and sucrose) were all bought from Yuanye Biotechnology Co., Ltd (Shanghai, China).
Acetonitrile
Manufactured by Yuanye Bio-Technology
Sourced in China
Acetonitrile is a colorless, flammable liquid commonly used as a solvent in various laboratory applications. It has a high polarity and low viscosity, making it a versatile solvent for a range of chemical reactions and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sucrose, by Yuanye Bio-Technology (2 mentions)
Phenol, by Yuanye Bio-Technology (2 mentions)
Sorbitol, by Yuanye Bio-Technology (1 mentions)
Folin ciocalteu reagent, by Yuanye Bio-Technology (1 mentions)
Fructose, by Yuanye Bio-Technology (1 mentions)
Glucose, by Yuanye Bio-Technology (1 mentions)
Glucose, by Chron Chemicals (1 mentions)
Methanol, by Yuanye Bio-Technology (1 mentions)
Sodium bicarbonate, by Chron Chemicals (1 mentions)
Formic acid, by Yuanye Bio-Technology (1 mentions)
3 5 dinitrosalicylic acid, by Yuanye Bio-Technology (1 mentions)
Ethanol, by Yuanye Bio-Technology (1 mentions)
L fucose, by Merck Group (1 mentions)
D arabinose, by Merck Group (1 mentions)
D glucuronic acid, by Merck Group (1 mentions)
L rhamnose, by Merck Group (1 mentions)
D xylose, by Merck Group (1 mentions)
D mannose, by Merck Group (1 mentions)
Potassium chloride (kcl), by Yuanye Bio-Technology (1 mentions)
Citric acid, by Yuanye Bio-Technology (1 mentions)
5 protocols using acetonitrile
1
Comprehensive Analysis of Fresh Apple
2
Polyphenol-Aroma Compound Analysis Protocol
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The polyphenol additive, primary aroma component standard, C7–C40 normal paraffin standard and phenolic acid standard utilized in this investigation were procured from PtSrTi (Chongqing, China).
The high-activity dry yeast utilized for fruit wine production was procured from Angel Yeast (Angel Yeast, Yichang, China). Chemical reagents such as Pectinase 500 U/mg, potassium metabisulfite, 3,5-dinitrosalicylic acid, phenol, methanol (HPLC), acetonitrile (HPLC), and formic acid (HPLC) were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Additionally, sodium bicarbonate, sodium potassium tartrate, DPPH (2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)) and glucose were acquired from Chengdu Kelong Chemical Reagent Factory (Chengdu., China).
The ABTS Assay Kit was procured from Suzhou Keming Biotechnology Co., Ltd. (Suzhou, China). The FRAP Assay Kit was procured from Suzhou Greis Biotechnology Co., Ltd. (Suzhou, China).
The high-activity dry yeast utilized for fruit wine production was procured from Angel Yeast (Angel Yeast, Yichang, China). Chemical reagents such as Pectinase 500 U/mg, potassium metabisulfite, 3,5-dinitrosalicylic acid, phenol, methanol (HPLC), acetonitrile (HPLC), and formic acid (HPLC) were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Additionally, sodium bicarbonate, sodium potassium tartrate, DPPH (2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)) and glucose were acquired from Chengdu Kelong Chemical Reagent Factory (Chengdu., China).
The ABTS Assay Kit was procured from Suzhou Keming Biotechnology Co., Ltd. (Suzhou, China). The FRAP Assay Kit was procured from Suzhou Greis Biotechnology Co., Ltd. (Suzhou, China).
3
Extraction and Characterization of Tamarind Seed Polysaccharides
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Tamarind seeds were collected from Yunnan, China. Monosaccharide standards (l -rhamnose, l -fucose, d -glucose, d -xylose, d -galactose, d -mannose, d -arabinose, d -glucuronic acid, and d -galacturonic acid) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ethanol, phenol, citric acid, boric acid, acetonitrile, NaNO3, trifluoroacetic acid TFA, HCl, H2SO4, NaOH, KBr, NaCl, KCl, CaCl2 and sucrose were purchased from Yuanye Bio-Technology Co. (Shanghai, China). All reagents used were of analytical grade unless otherwise specified.
4
Time-Dependent Evaluation of RRM and PRM
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RRM and PRMs that had been processed for 4, 8, 12, 18, 24 and 32 h were provided by Shanghai Dehua Traditional Chinese Medicine CO., Ltd., and the corresponding batch numbers were HSW2018051101-S, HSW2018051101-4H, HSW2018051101-8H, HSW2018051101-12H, HSW2018051101-18H, HSW2018051101-24H, and HSW2018051101-32H. The samples were authenticated by Professor Zhihai Huang, and voucher specimens were deposited in the Materials Medica Preparation Lab of the Second Affiliated Hospital of the Guangzhou University of Chinese Medicine.
Trans-2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (THSG), cis-THSG and polydatin were purchased from yuanye Bio-Technology Co., Ltd. Acetonitrile (No. H08J11E115101, P27A11P107214, T15A10F85743, purity ≥98%, Shanghai, China). Acetonitrile and methanol (HPLC grade), were supplied by E. Merck (Darmstadt, Germany), formic acid (HPLC grade) was purchased from fisher (United States), ultra-pure water was prepared by a Mili-Q water purification system (Millipore, MA, United States).
Trans-2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (THSG), cis-THSG and polydatin were purchased from yuanye Bio-Technology Co., Ltd. Acetonitrile (No. H08J11E115101, P27A11P107214, T15A10F85743, purity ≥98%, Shanghai, China). Acetonitrile and methanol (HPLC grade), were supplied by E. Merck (Darmstadt, Germany), formic acid (HPLC grade) was purchased from fisher (United States), ultra-pure water was prepared by a Mili-Q water purification system (Millipore, MA, United States).
5
Profiling Biogenic Amines in Soybeans
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The study material consisted of yellow soybeans [Glycine max (Linn.) Merr.], which were purchased from a market in Yantai city, Shandong province, China.
BAs or amine hydrochlorides, such as histamine (HIS), β-phenylethylamine (PHE), Tryptamine (TRP), cadaverine dihydrochloride (CAD), putrescine dihydrochloride (PUT), tyramine hydrochloride (TYR), spermine (SPM), and spermidine (SPD), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), with purities of ≥ 98%.
Acetone and acetonitrile were of HPLC grade, while the other chemicals were analytically pure. All chemicals were purchased from Sinopharm Group Co., Ltd. (Beijing, China), and the plate count agar (PCA, CM101), De Man Rogosa Sharpe agar (MRSA, CM188), Manitol salt agar (MSA, CP813), brain heart infusion agar (BHIA, CM918), potato dextrose agar (PDA, CM123, and yeast extract glucose chloramphenicol agar (YGC, CM840) were purchased from Land Bridge Technology Co., Ltd. (Beijing, China). Bacterial and yeast genome extraction kits and lyticase were purchased from Tiangen Biotech Co., Ltd. (Beijing, China), and the 2 × Taq master mix (P111-03) was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China).
BAs or amine hydrochlorides, such as histamine (HIS), β-phenylethylamine (PHE), Tryptamine (TRP), cadaverine dihydrochloride (CAD), putrescine dihydrochloride (PUT), tyramine hydrochloride (TYR), spermine (SPM), and spermidine (SPD), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), with purities of ≥ 98%.
Acetone and acetonitrile were of HPLC grade, while the other chemicals were analytically pure. All chemicals were purchased from Sinopharm Group Co., Ltd. (Beijing, China), and the plate count agar (PCA, CM101), De Man Rogosa Sharpe agar (MRSA, CM188), Manitol salt agar (MSA, CP813), brain heart infusion agar (BHIA, CM918), potato dextrose agar (PDA, CM123, and yeast extract glucose chloramphenicol agar (YGC, CM840) were purchased from Land Bridge Technology Co., Ltd. (Beijing, China). Bacterial and yeast genome extraction kits and lyticase were purchased from Tiangen Biotech Co., Ltd. (Beijing, China), and the 2 × Taq master mix (P111-03) was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China).
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