Lambda 10 3 filter changer

Manufactured by Sutter Instruments

The Lambda 10-3 is a filter changer designed for fast and precise switching between multiple filters. It can hold up to 10 filters and allows for quick and automated filter changes during experiments or imaging sessions.

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Lab products found in correlation

Ti e wide field fluorescence microscope, by Nikon (1 mentions) Ti e perfect focus system, by Oxford Instruments (1 mentions) Ixon3 emccd camera, by Oxford Instruments (1 mentions) Ti e widefield microscope, by Nikon (1 mentions) Ti e spinning disc confocal microscope, by Nikon (1 mentions) Myopacer cell stimulator, by IonOptix (1 mentions) Maxwell rsc instrument, by Promega (1 mentions)

Spelling variants of this product

Lambda 10-3 (15 citations) Lambda 10-3 Optical Filter Changer (3 citations) Lambda-10 (2 citations)

3 protocols using lambda 10 3 filter changer

Wide-field Fluorescence Microscopy Imaging

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Imaging experiments were performed on a Nikon Ti-E wide-field fluorescence microscope equipped with Nikon elements software, Ti-E perfect focus system, an iXon3 EMCCD camera (Andor), mercury arc lamp, and YFP FRET (434/16 excitation, 458 dichroic, 535/20 emission) and CFP (434/16 excitation, 458 dichroic, 470/24 emission) filter sets. External excitation and emission filter wheels were controlled by a Lambda 10–3 filter changer (Sutter Instruments), while dichroic mirrors were placed on cubes in the dichroic turret. Images were collected using a ×60 oil objective (numerical aperture 1.40), 100 ms exposure time, electron mutiplying gain 1 MHz at 16-bit readout mode with an electron mutiplying gain multiplier of 200, and a neutral density filter with 25% light transmission. Cells were maintained at 37 °C and 5% CO₂ in a LiveCell environment chamber (Pathology Devices) during the experiments. Images were collected every minute.

Nolin E., Gans S., Llamas L., Bandyopadhyay S., Brittain S.M., Bernasconi-Elias P., Carter K.P., Loureiro J.J., Thomas J.R., Schirle M., Yang Y., Guo N., Roma G., Schuierer S., Beibel M., Lindeman A., Sigoillot F., Chen A., Xie K.X., Ho S., Reece-Hoyes J., Weihofen W.A., Tyskiewicz K., Hoepfner D., McDonald R.I., Guthrie N., Dogra A., Guo H., Shao J., Ding J., Canham S.M., Boynton G., George E.L., Kang Z.B., Antczak C., Porter J.A., Wallace O., Tallarico J.A., Palmer A.E., Jenkins J.L., Jain R.K., Bushell S.M, & Fryer C.J. (2019). Discovery of a ZIP7 inhibitor from a Notch pathway screen. Nature chemical biology, 15(2), 179-188.

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Multimodal Imaging and Electrical Stimulation Protocol

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Samples for all imaging experiments were imaged on a Nikon Ti-E spinning disc confocal microscope equipped with Nikon Elements software, Ti-E perfect focus system, Yokogawa CSU-X1 spinning disc head, Andor 888 Ultra EMCCD camera and Oko Labs enclosed environmental chamber set at 37 °C.
Samples for each type of imaging experiment except immunofluorescence experiments were also imaged on a Nikon Ti-E widefield microscope equipped with Nikon Elements software, Ti-E perfect focus system, Andor iXon3 EMCCD camera, Sutter Instruments LD-LS/30 xenon arc lamp, and Sutter Instruments Lambda 10-3 filter changer.
For electrical stimulation, an IonOptix Myopacer cell stimulator was equipped with a custom set of platinum wire electrodes. Stimulations were performed with 30 V bipolar waveform 10 ms pulses at 5 Hz for a duration of 1 minute, unless specified otherwise.
RNA extraction for next-generation sequencing was performed using a Promega Maxwell RSC Instrument.
Quantitative PCR was performed using a BIO-RAD CFX384 Real Time PCR Detection System instrument.

Sanford L., Carpenter M.C, & Palmer A.E. (2019). Intracellular Zn2+ transients modulate global gene expression in dissociated rat hippocampal neurons. Scientific Reports, 9, 9411.

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Photoactivated RecBCD Reaction Monitoring

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Light from a Sutter Instruments LS 300W Xenon Arc Lamp, controlled by a Sutter
Instruments Lambda 10-3 filter changer and shutter, was passed through a 340- to 380-nm
bandpass filter and led to the sample via a liquid lightguide (3 mm diameter lightpath).
RecBCD reaction was started by addition of ATP, and the reaction mixtures (10–20
µl) were vortexed and centrifuged to the bottom of the 1.5-ml microfuge tube. The
end of the arc lamp’s lightguide was supported above the sample by the walls of
the tube, ~8 mm above the surface of the liquid. Light intensity, as measure by a
Coherent LasermateQ meter, was 5.7 mW/mm². The shutter was opened from 15 to 75
s after addition of ATP. Gel loading buffer was added and samples separated on gels.
Unirradiated controls were handled in parallel.

Taylor A.F., Amundsen S.K., Guttman M., Lee K.K., Luo J., Ranish J, & Smith G.R. (2014). Control of RecBCD Enzyme Activity by DNA Binding- and Chi Hotspot-Dependent Conformational Changes. Journal of molecular biology, 426(21), 3479-3499.

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