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Human il 1β

Manufactured by Elabscience

Human IL-1β is a recombinant protein that corresponds to the mature form of the human interleukin-1 beta (IL-1β) cytokine. IL-1β is a pro-inflammatory cytokine that plays a central role in the regulation of immune and inflammatory responses.

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3 protocols using human il 1β

1

Quantification of Inflammatory Cytokines

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Quantification of IL-1β and TNF-α in rat serum and cell culture supernatant was accomplished with human IL-1β (Elabscience, Wuhan, China) and TNF-α (mlbio, Shanghai, China) enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s protocols. Cytokine concentration was evaluated using a microplate reader (Thermo) at 450 nm.
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2

Quantifying Inflammatory Cytokines in Cell Culture

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Interleukin-1 beta (IL-1β), IL-6, IL-10, and TGF-β1 were detected in the culture supernatant using the following Elisa Kit: Human IL-1β (Interleukin 1 Beta) ELISA Kit (Catalog No. E-EL-H0149c, Elabscience), Human IL-6 (Interleukin 6) ELISA Kit (Catalog No. E-EL-H6156, Elabscience), Human IL-10 (Interleukin 10) ELISA Kit (Catalog No. E-EL-H6154, Elabscience), and TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit (Catalog No. E-EL-0162c, Elabscience). All steps conform to the manufacturer’s guidelines.
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3

Quantification of Inflammatory Mediators

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Human IL-1β (Cat: H0149c, Elabscience Biotech, Wuhan, China), IL-10 (Cat: E-EL-H0103c, Elabscience Biotech), TNF-α (Cat: E-EL-H0109c, Elabscience Biotech), CXCL1 (Cat: CSB-E09150h, Cusabio, Wuhan, China), CXCL2 (Cat: CSB-E07420h, Cusabio), CXCL8 (Cat: H6008, Elabscience Biotech), and HMGB-1 (Cat: E-EL-H1554, Elabscience Biotech) levels in CSF or cell culture supernatants were quantified using a commercial ELISA kit according to the manufacturer’s instructions.
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