Plasma IgG1 and IgG3 concentrations were determined using the IgG Subclass Human ELISA Kit (ThermoFisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. All plasma samples were diluted 1:2500 for both IgG1 and IgG3 quantifications.
Igg subclass human elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The IgG Subclass Human ELISA Kit is a laboratory equipment designed for the quantitative determination of human immunoglobulin G (IgG) subclasses in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the levels of IgG1, IgG2, IgG3, and IgG4 subclasses.
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3 protocols using igg subclass human elisa kit
1
Quantifying Plasma IgG1 and IgG3
2
Serum IgG Subclass Analysis in MS
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Serum was collected during diagnostic workup between 2010 and 2021. Most of the samples underwent instant diagnostic testing for IgG and IgM by turbidimetry (cobas 8000, Roche, Basel, Switzerland) and additional blood samples were stored for later analysis at −80°C in the biobank of the Department of Neurology. Frozen samples of MS patients or controls with missing data on Ig concentrations were thawed and analyzed with the same method and machine.
For a subset of patients, frozen sera were available in our biobank (inclusion criteria maximum time between measurement of IgG and IgM and blood sampling for further analysis of IgG subclasses 6 months) to test for IgG subclasses 1–4 using a commercially available ELISA kit (IgG Subclass Human ELISA kit, Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. Each sample was tested in double wells.
For a subset of patients, frozen sera were available in our biobank (inclusion criteria maximum time between measurement of IgG and IgM and blood sampling for further analysis of IgG subclasses 6 months) to test for IgG subclasses 1–4 using a commercially available ELISA kit (IgG Subclass Human ELISA kit, Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. Each sample was tested in double wells.
3
Quantitative ELISA for Heat-Exposed Antibodies
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ELISA was used to determine the Ab activity after heat exposure.
Depending on the type of Abs, commercially available ELISA kits including
an IgG (Total) human uncoated ELISA kit and an IgG subclass human
ELISA kit (Thermo Fisher, USA) were used. Plates were read at 450
nm on a BioTek Synergy HT plate reader using BioTek Gen5 (BioTek).
Data are representative of an average of three sample replicates and
normalized to the stock or initial Abs activity after vacuum drying.
Depending on the type of Abs, commercially available ELISA kits including
an IgG (Total) human uncoated ELISA kit and an IgG subclass human
ELISA kit (Thermo Fisher, USA) were used. Plates were read at 450
nm on a BioTek Synergy HT plate reader using BioTek Gen5 (BioTek).
Data are representative of an average of three sample replicates and
normalized to the stock or initial Abs activity after vacuum drying.
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