Experiments were performed using BMVECs isolated from normal Wistar and type 2 diabetic Goto-Kakizaki (GK) rats as reported previously (14 (link), 16 (link)). Both Wistar and GK cells were grown in endothelial growth media (VEC technologies, Rensselaer, NY, USA) and plated on flasks coated with 0.1% gelatin. Cells were grown to 80–90% confluency and passage 5–8 were used in current experiments. Cells were serum starved in Dulbecco’s Modified Eagle’s medium (DMEM) for 2 h and incubated with/without Iron (III) sulfate (0.1 mM; Sigma Aldrich) or DFX (100μM; Sigma Aldrich) or combination of both for 6 h in 2% FBS containing DMEM. Cell lysates were collected and prepared for Western blot analysis, lipid-peroxidation measurements, total glutathione measurements and immunofluorescence measurement.
Iron 3 sulfate
Manufactured by Merck Group
Sourced in United States
Iron (III) sulfate is a chemical compound with the formula Fe2(SO4)3. It is a yellowish-brown crystalline solid that is soluble in water. The primary function of Iron (III) sulfate is as a coagulant and flocculant in water treatment processes, where it aids in the removal of suspended particles and impurities from water.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Iron 3 chloride, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Trolox, by Merck Group (1 mentions)
Horseradish peroxide, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine, by Merck Group (1 mentions)
Thiobarbituric acid tba, by Merck Group (1 mentions)
Dichlorodihydrofluorescein diacetate assay dcfh da, by Merck Group (1 mentions)
Itlc sg strip, by Agilent Technologies (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
L dopa, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
5 protocols using iron 3 sulfate
1
Oxidative Stress Response in Diabetic Endothelial Cells
2
Antioxidant Evaluation of Fibroblast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals have an analytical purity and were used without further purification. Iron(III) chloride (FeCl3·6H2O), iron(III) sulfate (FeSO4), trolox, H2O2, horse radish peroxide, phosphate buffer pH 7, Folin-Ciocalteu reagent, DPPH (2,2-diphenyl-1-picrylhydrazyl), 2,4,6-tripyridyl-s-triazine (TPTZ), trichloroacetic acid (TCA), thiobarbituric acid (TBA), sodium dodecyl sulphate (DSD) and dichlorodihydrofluorescein diacetate assay (DCFH-DA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ethanol and methanol were bought from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM) containing glucose at 4.5 mg/mL (25 mM), penicillin, streptomycin, trypsin–EDTA, fetal bovine serum (FBS) and phosphate buffered saline (PBS) without Ca and Mg were purchased from Gibco (San Diego, USA). DTNB (dithiobis-2-nitrobenzoic acid, Ellman's reagent) was provided by Serva Electrophoresis GmbH (Heidelberg, Germany). The fibroblasts cell line was obtained from American Type Culture Collection (ATCC).
3
Radiolabeling of p-NCS-Bz-MANOTA Construct
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Iron(III) sulfate (97%),
ammonium iron(II) sulfate hexahydrate (99%), sodium hydroxide (99%),
LDOPA (98%), N-hydroxysuccinimide (98%) N,N-diisopropylethylamine (DIEA) (99.5%), and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride were purchased from Sigma-Aldrich. Demineralized water
(conductivity, σ = 2.2 μS cm–1) was used for the hydrothermal synthesis. Phosphate buffered saline
(PBS) 1× solution was purchased from Fisher Bioreagents. Extra
dry dimethyl sulfoxide (DMSO > 99.7%) was purchased from
Acros. MeO–PEG–NH2 (molecular weight, MW
= 2000 Da) was purchased from Iris Biotech GmbH. 2,2,2-(2-{[2-(4-Isothiocyanatophenyl)acetamido]methyl}-1,4,7-triazacyclononane-1,4,7-triyl)triacetic
acid (p-NCS-Bz-MANOTA) was synthesized as previously
described.42 (link)64Cu copper chloride
(64CuCl2, 1 in 0.1 N HCl) was purchased from
Arronax (Saint-Herblain, France). The radiolabeling yield and the
absence of free 64Cu in the labeled constructs were determined
using instant thin layer chromatography impregnated with silica gel
(ITLC-SG) strips (Agilent, Santa-Clara, CA).
ammonium iron(II) sulfate hexahydrate (99%), sodium hydroxide (99%),
LDOPA (98%), N-hydroxysuccinimide (98%) N,N-diisopropylethylamine (DIEA) (99.5%), and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride were purchased from Sigma-Aldrich. Demineralized water
(conductivity, σ = 2.2 μS cm–1) was used for the hydrothermal synthesis. Phosphate buffered saline
(PBS) 1× solution was purchased from Fisher Bioreagents. Extra
dry dimethyl sulfoxide (DMSO > 99.7%) was purchased from
Acros. MeO–PEG–NH2 (molecular weight, MW
= 2000 Da) was purchased from Iris Biotech GmbH. 2,2,2-(2-{[2-(4-Isothiocyanatophenyl)acetamido]methyl}-1,4,7-triazacyclononane-1,4,7-triyl)triacetic
acid (p-NCS-Bz-MANOTA) was synthesized as previously
described.42 (link)64Cu copper chloride
(64CuCl2, 1 in 0.1 N HCl) was purchased from
Arronax (Saint-Herblain, France). The radiolabeling yield and the
absence of free 64Cu in the labeled constructs were determined
using instant thin layer chromatography impregnated with silica gel
(ITLC-SG) strips (Agilent, Santa-Clara, CA).
4
Synthesis of Polypyrrole Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bismuth (99.99%, Vital Materials, Guangzhou, China) and tellurium granules (99.9%, Vital Materials, Guangzhou, China) with pyrrole (98%, Sigma-Aldrich, St. Louis, MO, USA) were used as raw materials. Chemical oxidation of pyrrole was conducted by using Iron(III) sulfate (97%, Sigma-Aldrich, St. Louis, MO, USA). Each chemical was used with no further purification.
5
Antioxidant Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study has used analytical grade ethyl acetate (EtOAc), n-hexane, and methanol (MeOH), Quercetin (Sigma Aldrich, India), DPPH (1, 1-diphenyl-2picrylhydrazyl) (Sigma Aldrich, Germany), TPTZ (2,4,6-tripyridylstriazine) (Sigma Aldrich, Switzerland), Iron (II) sulfate (pa, sigma-aldrich), Iron (III) sulfate (pa, sigmaaldrich), Tween 20 (pa, sigma-aldrich), FeCl3, HCl, sodium acetate, and acetic acid (glacial) (Merck, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!