EZ-Magna RIP kit was used. Cells were broken using RIPA buffer and probed with anti-YTHDF2, anti-IgG or anti-Ago2 antibodies, or NC IgG (Abcam, USA). Precipitated RNA was measured by qPCR.
Nc igg
Manufactured by Abcam
Sourced in United States
NC IgG is a control antibody used to assess non-specific binding in immunoassays. It is a non-specific immunoglobulin G (IgG) antibody derived from normal serum.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using nc igg
1
RNA Immunoprecipitation and qPCR Analysis
2
Determining 1700020I14Rik-RISC Association
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RIP assay was performed according to the literature [35 (link)] to determine the association between 1700020I14Rik and the RISC. The cell lysate was incubated with RIP immunoprecipitation buffer, which contained magnetic beads conjugated with Ago2 antibody (Abcam, USA) and NC IgG (Abcam, Cambridge, USA). RIP experiments were performed using Magna RIP kit (Millipore, USA). Co-precipitated RNAs were detected via qRT-PCR.
3
Detecting RNA-Protein Interactions by RIP
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The RNA Immunoprecipitation (RIP) assay was performed using the Magna RIP kit (Millipore, Billerica, MA, USA) in accordance with the manufacturer’s instructions. For immunoprecipitation, whole-cell lysates were incubated with RIP immunoprecipitation buffer containing the Ago2 antibody (Abcam, Burlingame, CA, USA) and NC IgG (Abcam, Cambridge, MA, USA)-conjugated magnetic beads. After 2 h of incubation, precipitated RNA was purified to detect the expression of MEG3 and miR-181a through qRT-PCR [84 (link)].
4
Ago2-based RIP for RNA Analysis
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RIP experiments were performed using Magna RIP kit (Millipore, Billerica, MA, USA). The cell lysate was incubated with RIP immunoprecipitation buffer, which contained magnetic beads conjugated with Ago2 antibody (Abcam, Burlingame, CA, USA) and NC IgG (Abcam, Cambridge, MA, USA). qRT-PCR analysis was conducted after co-precipitate.
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