The specific DOT1L inhibitor EPZ-5676 was obtained from Chemietek. Lithium chloride and EX527 were purchased from Sigma, XAV-939 from Selleck and SRT1720 from Calbiochem. HiPerFect and all siRNAs except DOT1L siRNA were purchased from Qiagen (Supplementary Table 2 ). DOT1L siRNA and lipofectamine RNAimax were purchased from Invitrogen. Recombinant human WNT3A protein was purchased from R&D Systems.
Dot1l sirna
Manufactured by Thermo Fisher Scientific
DOT1L siRNA is a laboratory reagent that can be used to knock down the expression of the DOT1L gene. DOT1L is a methyltransferase enzyme that plays a role in the regulation of gene expression. The DOT1L siRNA can be used in cell-based experiments to study the effects of DOT1L knockdown.
Automatically generated - may contain errors
Lab products found in correlation
Lithium chloride (licl), by Merck Group (1 mentions)
Xav939, by Selleck Chemicals (1 mentions)
Epz 5676, by Chemietek (1 mentions)
Recombinant human wnt3a protein, by R&D Systems (1 mentions)
Ex 527, by Merck Group (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Hiperfect, by Qiagen (1 mentions)
H3k36me2, by Cell Signaling Technology (1 mentions)
Sirna, by Roche (1 mentions)
H4k20me3, by Cell Signaling Technology (1 mentions)
Mda mb 231, by Roche (1 mentions)
Sirna, by Thermo Fisher Scientific (1 mentions)
H3k27me3, by Cell Signaling Technology (1 mentions)
H3k4me3, by Cell Signaling Technology (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
X tremegene transfection reagent, by Roche (1 mentions)
Mda mb 157, by American Type Culture Collection (1 mentions)
Hcc70, by American Type Culture Collection (1 mentions)
2 protocols using dot1l sirna
1
Inhibition and Knockdown of DOT1L in Cell Lines
2
Targeting DOT1L in Breast and Lung Cancer Cell Lines
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Human cell lines BT549, MDA-MB231, MCF-7, MDA-MB157, HCC70, A549 and NB4 were obtained from the American Type Culture Collection (ATCC) and cultured using ATCC recommended conditions. Primary antibodies against H3K79me2, H3K27me3, H3K4me3, H3K36me2, H4K20me3, H3K9me-pan were purchased from Cell Signaling (Danvers, MA). siRNA experiments were performed by transfecting MDA-MB-231 cells with X-tremeGENE Transfection Reagent (Roche) and 10 nM siRNA (Invitrogen), according to the manufacturer's protocol. The sequences for DOT1L siRNA (Invitrogen) were:
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