Immuno-detection on the membranes [18 (link),19 (link)] was with primary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) against myostatin (GDF8/11, mouse monoclonal antibody SC-393335); interleukin 6 (IL-6, rabbit polyclonal antibody SC-1265-R); and housekeeping beta-actin (mouse monoclonal, followed by secondary antibodies: anti-mouse IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology, Danvers, MA, USA), or anti-rabbit IgG linked to HRP (Amersham GE, Pittsburgh, PA, USA). Bands were visualized using luminol (SuperSignal West Pico; Chemiluminescent, Pierce, Rockford, IL, USA). For negative controls, the primary antibody was omitted. Densitometric analysis was performed in certain cases as stated, correcting by the housekeeping proteins.
Sc 393335
Manufactured by Cell Signaling Technology
Sourced in United States
SC-393335 is a laboratory reagent used for research purposes. It is a small molecule that acts as a proteasome inhibitor. The core function of this product is to inhibit the activity of the proteasome, a multi-subunit protein complex responsible for the degradation of unwanted or damaged proteins within cells. This product can be utilized in various research applications to study cellular processes related to protein homeostasis and turnover.
Automatically generated - may contain errors
2 protocols using sc 393335
1
Western Blot Analysis of Myostatin, IL-6, and β-Actin
2
Western Blot Analysis of Myostatin, Oct4, and PCNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immuno-detection on the membranes was with primary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) against myostatin (GDF8/11, mouse monoclonal antibody SC-393335); and housekeeping beta-actin (mouse monoclonal, followed by secondary antibodies: anti-mouse IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling Technology, Danvers, MA, USA), or anti-rabbit IgG linked to HRP (Amersham GE, Pittsburgh, PA, USA). Oct4 was estimated by a polyclonal antibody from Biovision catalog 3576, at 1:500; and PCNA with a monoclonal antibody from Millipore MAB424, at 1:2000. Bands were visualized using luminol (SuperSignal West Pico; Chemiluminescent, Pierce, Rockford, IL, USA). For negative controls, the primary antibody was omitted. Densitometric analysis was performed in certain cases, as stated, correcting by the housekeeping proteins [5 (link),7 (link),8 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!