Relatively accurate sequenced and assembled data are required for further analyses, such as sequence annotation, variation detection and expression analyses. Therefore, twelve unigenes were randomly selected, and the primer pairs for the RT-PCR amplification were designed and synthesized. The leaf cDNA samples of D. jenkinsiana were used as a template for the RT-PCR, and the PCR products at the expected product size were subcloned into a T-easy vector and then sequenced. The sequence alignment analysis was performed with DNASTAR (version 7.10 and http://www.dnastar.com ) with default parameters.
Version 7
Manufactured by DNASTAR
Sourced in United States
DNASTAR version 7.10 is a software suite for bioinformatics analysis. It provides tools for sequence assembly, alignment, and analysis. The software is designed to support research in areas such as genomics, transcriptomics, and molecular biology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using version 7
1
Unigene Sequencing and Validation
2
Comparative Analysis of PPV7 Protein Sequences
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NS1 and Cap amino acid sequences were derived from 59 PPV7 genomes. Sequence identity was analyzed and aligned with the Clustal W method of the MegAlign program of DNASTAR, version 7.10 (Lasergene) (DNASTAR, Inc., Wisconsin, USA). Amino acid sequences were aligned with the ESPript 3.0 online tool (ESPript 3.0, http://espript.ibcp.fr/ESPript/ESPript/ , 4 July 2020).
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