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Model 500 ultrasonic dismembrator

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Model 500 Ultrasonic Dismembrator is a laboratory instrument designed for the disruption and homogenization of biological samples. It utilizes high-frequency sound waves to agitate and break down cellular structures, enabling efficient sample preparation for downstream analysis.

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Lab products found in correlation

Analog vortex mixer, by Avantor (1 mentions) Microfluidizer m110s, by Microfluidics (1 mentions) V3 chemistry kit, by Illumina (1 mentions) Nebnext ultra dna library prep kit, by Illumina (1 mentions) Hiseq 2000, by Illumina (1 mentions) Nanosizer, by Malvern Panalytical (1 mentions)

4 protocols using model 500 ultrasonic dismembrator

1

Cellular Fractionation and Protein Extraction

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Cells were plated at density of 3 × 106 cells in 15 cm2 dishes. The following day, cells were treated with 200 nM MMC for 24 h. Cells were harvested and resuspended in ice-cold PBS. A portion of the pellet was retained as a whole cell lysate (W). The remaining pellet was lysed on ice in cytoskeletal buffer (CSK) (300 mM Sucrose, 100 mM NaCl, 3 mM MgCl2, 0.5% v/v Triton-X-100, 1 mM EGTA, 10 mM PIPES, pH 6.8). The supernatant, containing soluble cytoplasmic and nuclear proteins, was collected as the soluble fraction (S). The remaining pellet, containing chromatin-associated and nuclear insoluble proteins (C), and the whole-cell lysate pellet, were lysed in 2% SDS lysis buffer with sonication for 10 s at 10% amplitude using a Fisher Scientific Model 500 Ultrasonic Dismembrator.
Vierra D.A., Garzon J.L., Rego M.A., Adroved M.M., Mauro M, & Howlett N.G. (2017). Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure. Oncotarget, 8(44), 76443-76457.
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2

Microfluidized Kolliphor EL/Pluronic P105 Emulsions

Cited 1 time
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Preparation of 3% w/w Kolliphor EL/ 2% w/w Pluronic P105 surfactant solution is reported previously (Patel S. K. et al. 2013 ). Emulsification protocol developed previously (Janjic et al. 2008 (link); O’Hanlon et al. 2012 (link)) was partially modified. First, surfactant solution was added to the oil(s). If present, solubilizer or PFPE-tyramide dissolved in transcutol (at 50 mg/mL) was added to the mixture. Then, coarse emulsions were produced with an analog vortex mixer (VWR, Radnor, PA) on high for 30 seconds. Triphasic emulsions were sonicated for 30 seconds at 29% amplitude with Model 500 Ultrasonic Dismembrator (FisherScientific, Pittsburgh, PA). Microfluidization was performed on all coarse emulsions by Microfluidizer M110S (Microfluidics Corp., Westwood, MA) at 15,000 psi liquid pressure for 20 pulses over ice cold interaction chamber. Emulsions were stored at 4 °C in glass vials. This method was used to produce all presented formulations. Microfluidization processing parameters were kept constant throughout the study.
Lambert E, & Janjic J.M. (2019). Multiple Linear Regression Applied to Predicting Droplet Size of Complex Perfluorocarbon Nanoemulsions for Biomedical Applications. Pharmaceutical development and technology, 24(6), 700-710.
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3

DNA Library Preparation and Illumina Sequencing

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The DNAs of M1, F1 and F’-Mix, three samples were built into libraries and used for sequencing. Each DNA sample was separately sonicated using a Fisher Scientific Model 500 Ultrasonic Dismembrator. DNAs were manually size-selected into 300-500 bp fragments using gel electrophoresis, and libraries were prepared according to the methodology originally described in the NEBNext Ultra DNA Library Prep Kit from Illumina. Three libraries were quantified by realtime fluorescence quantitative PCR, and run in three lanes on Illumina HiSeq 2000 using 150 base PE reads (v3 chemistry kit, Illumina, USA). Illumina sequencing and library preparation was performed by the Beijing Genomics Institute.
Ou M., Yang C., Luo Q., Huang R., Zhang A.D., Liao L.J., Li Y.M., He L.B., Zhu Z.Y., Chen K.C, & Wang Y.P. (2017). An NGS-based approach for the identification of sex-specific markers in snakehead (Channa argus). Oncotarget, 8(58), 98733-98744.
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4

Nanoparticle Size Characterization by Nanosizer

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The Nanosizer, by Malvern, United Kingdom, was employed to determine the mean diameter (Dp) and degree of size variation (DPI) of precipitated PS nanoparticles. The measurements were performed at a temperature of 25 °C, with a reading angle of 90° and a wavelength of 633 nm. The obtained PS particles were dispersed in distilled water and then exposed to sonication using a Model 500 Ultrasonic Dismembrator from Fisher Scientific, USA, to break up any present aggregates (de Sousa Cunha et al. 2021 (link)).
El-Kurdi N., El-Shatoury S., ElBaghdady K., Hammad S, & Ghazy M. (2024). Biodegradation of polystyrene nanoplastics by Achromobacter xylosoxidans M9 offers a mealworm gut-derived solution for plastic pollution. Archives of Microbiology, 206(5), 238.
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