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Eclipse ti2

Manufactured by Hamamatsu Photonics

The Eclipse Ti2 is an inverted research microscope system designed for advanced live-cell imaging applications. It features a high-precision stage, advanced optical components, and a modular design that allows for customization to meet the specific needs of researchers. The Eclipse Ti2 provides a stable and reliable platform for a wide range of imaging techniques, including fluorescence, phase contrast, and brightfield microscopy.

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3 protocols using eclipse ti2

1

Microswimmers Imaging and Tracking

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The microswimmers experiments were performed in a simple cell consisting of a Teflon ring of 15 mm innner diameter and 4 mm height glued on a microscope slide with NOA 63 UV glue from Norlands optics. Stock H2O2 (30 v/v%, Acros Organics) was added to dilute particle suspensions of the Pt-silica Janus raspberry particles to obtain 300 μL of a fuel rich (3 v/v% H2O2) solution, which was then pipetted into the Teflon ring before closing the cell by placing a glass coverslip on top. 2044 × 2048 pixels images were recorded in an inverted microscope (Nikon Eclipse Ti2) using a 60x objective and a Hamamatsu Orca Flash 4.0 v3 camera at 67 fps.
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2

Liquid-Liquid Phase Separation of MST2-SAV1 Complexes

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MST2:SAV1 complexes were diluted in 10 mM Tris, pH 8.5, 400 mM NaCl and 5 mM βME to the concentrations indicated in the presence or absence of 5% (w/v) 1,6‐hexanediol, incubated for 2 h and 7.5 μL loaded into a chamber made from clean glass slide with a 120‐μm double‐sided sticker (Grace Biolabs). Two micrometer Z sections of each well were taken on a Nikon Eclipse Ti2 using a Hamamatsu digital camera C13440 with a 60× air‐immersion lens. ImageJ was used to compile the Z‐stacks and calculate the total area corresponding to the droplets. Data were graphed and analysed in Prism (GraphPad, La Jolla, California).
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3

Imaging Diluted Particle Suspensions

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Diluted particle suspensions were imaged inside a glass channel with an inner cross-section of 100 μm × 1 mm and a length of 5 cm. In order to provide a mechanical support during imaging, the channel was glued to a microscope slide with the two ends protruding from the slide. The inlet of the channel was inserted in a tube (Tygon 2001 tubing ID 0.64 mm) and glued with (2 × 15 mL, 5 min rapid glue) from Araldite. The suspension was injected with a 1 mL syringe (Injekt from B. Braun Melsungen AG) using a syringe pump (NE-1000 from New Era Pump Systems Inc.). Images of the setup can be found in Fig. SI2-A, ESI. 1336 × 726 pixels images were recorded in an inverted microscope (Nikon Eclipse Ti2) using a 100x oil objective and a Hamamatsu Orca Flash 4.0 v3 camera at 67 fps.
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