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Human th1 th2 cytokine procartaplex panel

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The Human TH1/TH2 Cytokine ProcartaPlex Panel is a multiplex assay that allows for the simultaneous quantitative measurement of multiple human cytokines in a single sample. The panel measures the levels of specific cytokines associated with T-helper 1 (Th1) and T-helper 2 (Th2) immune responses.

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Human magnetic luminex assay, by R&D Systems (2 mentions) Tgf β and ifn γ, by BD (2 mentions)

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ProcartaPlex panels (2 citations)

6 protocols using human th1 th2 cytokine procartaplex panel

1

Cytokine Secretion Analysis

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Cytokine secretion was analyzed in the supernatant as indicated in the figures using the eBioscience™ ProcartaPlex Human Th1/Th2 Cytokine-Panel (Thermofisher) according to the manufacturer´s instructions.
Heine A., Held S.A., Daecke S.N., Flores C, & Brossart P. (2023). Spoilt for choice: different immunosuppressive potential of anaplastic lymphoma kinase inhibitors for non small cell lung cancer. Frontiers in Immunology, 14, 1257017.
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2

Plasma Cytokine Quantification Protocol

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The plasma levels of proinflammatory cytokines, including interleukin-2, tumor necrosis factor–α, and interferon-γ (IFN-γ), were measured using the ProcartaPlex Human Th1/Th2 Cytokine Panel (Thermo Fisher Scientific) according to the manufacturer's instructions.
Chen S.J., Chen C.C., Liao H.Y., Lin Y.T., Wu Y.W., Liou J.M., Wu M.S., Kuo C.H, & Lin C.H. (2022). Association of Fecal and Plasma Levels of Short-Chain Fatty Acids With Gut Microbiota and Clinical Severity in Patients With Parkinson Disease. Neurology, 98(8), e848-e858.
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3

Protein Quantification in Human ALF

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Protein levels in human ALF were determined using custom Human Magnetic Luminex Assays (R&D Systems), Human TH1/TH2 Cytokine ProcartaPlex Panel (ThermoFisher Scientific), and by Human SP-A, SP-D and C3 ELISA (LifeSpan Biosciences, Inc., Seattle, WA) per kit instructions.
Scordo J., Olmo-Fontánez A., Kelley H., Sidiki S., Arcos J., Akhter A., Wewers M, & Torrelles J. (2019). The Human Lung Mucosa Drives Differential Mycobacterium tuberculosis Infection Outcome in the Alveolar Epithelium. Mucosal immunology, 12(3), 795-804.
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4

Quantifying Cytokine Levels in M.tb-Infected Cells

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Protein levels in supernatants from exposed-M.tb infected ATs at 5 DPI were determined using: 1) Human enzyme-linked immunosorbent assay (ELISA) kits for TNF, IL-6, IL-8, CCL2, IL-1β, IL-12p40 and IL-10 (R&D Systems, Minneapolis, MN) and TGF-β and IFN-γ (BD Biosciences, San Jose, CA) and 2) Human TH1/TH2 Cytokine ProcartaPlex Panel (ThermoFisher Scientific, Waltham, MA), per kit instructions. ProcartaPlex analysis was performed using MasterPlex (Hitachi Solutions America) version 2.0.0.80 software.
Scordo J., Olmo-Fontánez A., Kelley H., Sidiki S., Arcos J., Akhter A., Wewers M, & Torrelles J. (2019). The Human Lung Mucosa Drives Differential Mycobacterium tuberculosis Infection Outcome in the Alveolar Epithelium. Mucosal immunology, 12(3), 795-804.
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5

Quantifying Cytokine Levels in M.tb-Infected Cells

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Protein levels in supernatants from exposed-M.tb infected ATs at 5 DPI were determined using: 1) Human enzyme-linked immunosorbent assay (ELISA) kits for TNF, IL-6, IL-8, CCL2, IL-1β, IL-12p40 and IL-10 (R&D Systems, Minneapolis, MN) and TGF-β and IFN-γ (BD Biosciences, San Jose, CA) and 2) Human TH1/TH2 Cytokine ProcartaPlex Panel (ThermoFisher Scientific, Waltham, MA), per kit instructions. ProcartaPlex analysis was performed using MasterPlex (Hitachi Solutions America) version 2.0.0.80 software.
Scordo J., Olmo-Fontánez A., Kelley H., Sidiki S., Arcos J., Akhter A., Wewers M, & Torrelles J. (2019). The Human Lung Mucosa Drives Differential Mycobacterium tuberculosis Infection Outcome in the Alveolar Epithelium. Mucosal immunology, 12(3), 795-804.
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6

Protein Quantification in Human ALF

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Protein levels in human ALF were determined using custom Human Magnetic Luminex Assays (R&D Systems), Human TH1/TH2 Cytokine ProcartaPlex Panel (ThermoFisher Scientific), and by Human SP-A, SP-D and C3 ELISA (LifeSpan Biosciences, Inc., Seattle, WA) per kit instructions.
Scordo J., Olmo-Fontánez A., Kelley H., Sidiki S., Arcos J., Akhter A., Wewers M, & Torrelles J. (2019). The Human Lung Mucosa Drives Differential Mycobacterium tuberculosis Infection Outcome in the Alveolar Epithelium. Mucosal immunology, 12(3), 795-804.
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