The cells (1.0 × 105 cells/mL) were cultured in a serum-free medium (RPMI1640 without FBS) for 24 hours and the culture medium was collected for measurements of LMW-HA concentrations. The culture medium sample was centrifuged at 14000 g for 10 minutes through Amicon Ultra-0.5 Centrifugal Filter Devices (MilliporeSigma, Darmstadt, Hessian, Germany) with a 100 kDa cutoff and the LMW-HA (<100 kDa) were collected [8 (link), 13 (link)]. The concentration of LMW-HA was measured using Quantikine ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, MN, USA). Assays were triplicated and the average concentrations were determined.
Quantikine elisa hyaluronan immunoassay
Manufactured by R&D Systems
Sourced in United States
The Quantikine ELISA Hyaluronan Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure hyaluronan levels in cell culture supernates, serum, plasma, and other biological fluids.
Automatically generated - may contain errors
5 protocols using quantikine elisa hyaluronan immunoassay
1
Quantification of LMW-HA in Cell Culture
2
Measurement of LMW Hyaluronan Concentration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells (1.0 × 105 cells/ml) were cultured in a serum-free medium (RPMI1640 without FBS) for 48 hours and the culture medium was collected for measurements of HA concentrations. The culture medium sample was centrifuged at 14000 g for 10 minutes through a Amicon Ultra-0.5 Centrifugal Filter Devices (MilliporeSigma, Darmstadt, Hessian, Germany) with a 100 kDa cutoff and the LMW-HA (<100 kDa) were collected [9 (link), 28 (link)]. The concentration of LMW-HA was measured using Quantikine ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, MN, USA). Assays were triplicated and the average concentrations were determined.
3
Quantification of Secreted Hyaluronan
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (1.0 × 105 cells/ml) were cultured in a serum-free medium (RPMI1640 without FBS) for 48 hours and the culture medium was collected for measurements of HA concentrations. The concentration of total HA was measured using the Quantikine ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, MN, USA). Assays were triplicated and the average concentrations were determined.
4
Quantitative Hyaluronan Immunoassay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of HA from both total, and 0.425 M NaCl plasma eluted fractions were determined using the Quantikine® ELISA Hyaluronan Immunoassay (R&D Systems Inc., MN, USA) according manufacturer’s instructions. This is a specific quantitative sandwich enzyme immunoassay designed to measure ≥35 kDa HA in human samples. Further details included as Supplementary Information.
5
Quantifying Hyaluronic Acid Secretion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HaCaT cells were seeded in 12-well plates at 1.5 × 105 cells/mL for 24 h. The cells were then exposed to the SSE ingredient (1 mg/mL) and incubated for 24 h. Cell culture supernatants were collected to quantify hyaluronic acid using the Quantikine™ ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. Additionally, cells were harvested and lysed for protein extraction using lysis buffer (50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 1% Nonidet-P40, and protease inhibitor cocktail tablets (Roche, Basel, Switzerland)). Total protein content was quantified using a Pierce™ BCA Protein Assay Kit (Thermo Fisher, MA, USA). All assays were performed in triplicate. The results are expressed as ngHA/mgprotein.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!