Quanta 200 feg

Manufactured by Thermo Fisher Scientific

Sourced in United States, Netherlands, Japan, France

The Quanta 200 FEG is a field emission gun scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a field emission electron source, providing high-brightness and small probe size for enhanced resolution and signal-to-noise ratio. The microscope is capable of operating in high vacuum, low vacuum, and environmental modes to accommodate different sample types and requirements.

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Lab products found in correlation

D8 advance, by Bruker (5 mentions) Jem 2100, by JEOL (4 mentions) Autosamdri 815b, by Tousimis (3 mentions) Lext ols4100, by Olympus (3 mentions) Tecnai g2 f30, by Thermo Fisher Scientific (3 mentions) Agilent 4294a, by Agilent Technologies (2 mentions) Uv 3600, by Shimadzu (2 mentions) Tensor 27, by Bruker (2 mentions) Xe 100e psia, by Thermo Fisher Scientific (2 mentions) T80 double beam spectrophotometer, by PG Instruments (2 mentions) Vertex 70 spectrometer, by Bruker (2 mentions) Sz61 stereomicroscope, by Olympus (2 mentions) D8 advance diffractometer, by Bruker (2 mentions) Tecnai g2 f30 tem, by Thermo Fisher Scientific (2 mentions) Jem 2010, by JEOL (2 mentions) X pert multi purpose, by Malvern Panalytical (2 mentions) Eclipse ti, by Nikon (2 mentions) 3view, by Ametek (2 mentions) Spectrum two, by PerkinElmer (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Quanta 200 (825 citations) Quanta 200 FEG SEM (33 citations) FEI Quanta 200 FEG (28 citations) Quanta 200 FEG microscope (12 citations) FEI Quanta 200 FEG SEM (8 citations) Quanta 200 FEG Environmental SEM (7 citations) Quanta 200 FEG MKII (5 citations) Tecnai FEG ESEM QUANTA 200 (4 citations) Quanta FEG-200 high resolution scanning electron microscope (3 citations) Quanta 200 FEG Field emission scanning electron microscope (3 citations)

217 protocols using quanta 200 feg

Characterizing Ceramic Membrane Microstructure

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Two types of ceramic membranes were prepared according to Paucar et al. [18 (link)]. The Peruvian clay ceramic membrane was made of clay obtained from the Department of Junín, Peru. The craft shop clay ceramic membrane was prepared using clay obtained from the Craft Shop of Idaho State University in Idaho, USA. These ceramic membranes were imaged on an FEI Quanta 200 FEG scanning electron microscope. To obtain an image of the material porosity, each ceramic disk was broken along one edge to obtain a flat surface and mounted to a microscope stub using silver paste. Disk fragments were coated with gold-palladium in an SPI sputter coater to minimize sample charging during imaging. Each membrane was imaged at low (~1.3 mm HFW [horizontal field width]) and high (~200–400 µm HFW) magnification. Secondary and backscattered image inputs were combined in a composite image to better resolve both the surface texture and porosity. After careful observation of SEM images (which are presented in the discussion section), it was decided that Peruvian clay ceramic membrane be used as a separator in this study.

Sato C., Apollon W., Luna-Maldonado A.I., Paucar N.E., Hibbert M, & Dudgeon J. (2023). Integrating Microbial Fuel Cell and Hydroponic Technologies Using a Ceramic Membrane Separator to Develop an Energy–Water–Food Supply System. Membranes, 13(9), 803.

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MC3T3-E1 Cell Seeding and SEM Characterization

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The established MC3T3-E1 line of murine pre-osteoblastic cells was obtained from ATCC and cultured in α-MEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (Gibco, BRL, USA) in a humidified 5% CO₂ balanced-air incubator at 37 ºC, with the medium being changed every other day. The scaffolds were seeded with 1.0×10⁵ MC3T3-E1 cells suspended in 100 μl of complete medium, and incubated for 4 h to permit cell attachment. The cell seeded scaffolds were then transferred to a 24-well plate containing 2 ml of complete medium per well. The control group consisted of the same number of cells seeded in wells containing 2 ml of α-MEM medium. At culture intervals of 3, 7 and 14 days, glass scaffolds with attached cells were removed, rinsed twice with warm phosphate-buffered saline (PBS), and soaked overnight in 2.5% glutaraldehyde in PBS. The fixed samples were then rinsed with PBS three times and dehydrated with a graded tetra butyl alcohol series. The samples were allowed to dry in air for 12 h at room temperature, sputter-coated with Au/Pd, and then observed using SEM (Quanta 200 FEG, FEI Co., The Netherlands) at 5 kV accelerating voltage.

Jia W., Lau G.Y., Huang W., Zhang C., Tomsia A.P, & Fu Q. (2018). Cellular Response to 3-D Printed Bioactive Silicate and Borosilicate Glass Scaffolds. Journal of biomedical materials research. Part B, Applied biomaterials, 107(3), 818-824.

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Comprehensive Material Characterization

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The elemental compositions of the samples were determined by energy dispersive X-ray spectroscopy (EDS) analysis conducted on an FEI Quanta 200 FEG environmental scanning electron microscope (SEM). X-ray diffraction (XRD) measurements were performed on the crystals using an X-ray diffractometer (Ultima III Rigaku, Cu-K_α radiation as an X-ray source). The scanning rate of 3° per minute and 2θ scanned from 10° to 70° were used to collect XRD data. Raman spectra were taken by a backscattering geometry on a LabRam HR800 Microscope system (Horiba Jobin Yvon), using the 633 nm a He-Ne laser as an optical source. Standard four-probe technique was used for resistivity and Hall-effect measurements on a Quantum Design PPMS-9.

Lv Y.Y., Cao L., Li X., Zhang B.B., Wang K., Bin Pang B.P., Ma L., Lin D., Yao S.H., Zhou J., Chen Y.B., Dong S.T., Liu W., Lu M.H., Chen Y, & Chen Y.F. (2017). Composition and temperature-dependent phase transition in miscible Mo1−xWxTe2 single crystals. Scientific Reports, 7, 44587.

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Scanning Electron Microscopy of Arabidopsis Cuticular Wax

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To visualize the alteration in cuticular wax content, a Quanta 200 FEG (FEI) scanning electron microscope was used to analyze the images of Arabidopsis plant leaves without any form of preparation [71 (link)]. The adaxial side of 4-week-old rosette leaves of GmSHN1-OE1, GmSHN9-OE1, and WT plants were collected and directly placed onto the stub. The accelerating voltage of the ESEM was 15 kV.

Xu Y., Wu H., Zhao M., Wu W., Xu Y, & Gu D. (2016). Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis. International Journal of Molecular Sciences, 17(4), 587.

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Scanning Electron Microscopy of Composite Cross-section

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The microscopic morphology of the composite cross-section was studied using a scanning electron microscope (FEI Quanta200 FEG, Eindhoven, The Netherlands).

Xu Z., Zhang C., Li Y., Zou J., Li Y., Yang B., Hu R, & Qian Q. (2023). Effect of the alumina micro-particle sizes on the thermal conductivity and dynamic mechanical property of epoxy resin. PLOS ONE, 18(10), e0292878.

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Characterization of Potassium Tantalate Niobate Thin Films

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The crystalline phase detection was performed by X-ray diffraction (XRD, Quanta 200FEG, FEI, America) using Cu Kα radiation. The morphology of KTN particles and films' cross-section was observed by scanning electron microscopy (SEM, S-4700, Hitachi, Japan). The films were plated Al on both sides to investigate the dielectric properties with the impedance analyzer (Agilent 4294A, America). The breakdown strength was tested on the dielectric withstand voltage test (HT-50, Guilin Electrical Equipment Scientific Research Institute, China) under the direct current voltage of 200 V s⁻¹.

Lin J., Li Y., Liu X., Li Y., Zheng W, & Yang W. (2020). Effect of the polarity of KTa1−xNbxO3 on the dielectric performance of the KTN/PVDF nanocomposites. RSC Advances, 10(44), 26256-26261.

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SEM Imaging of E. coli with GO-Ag

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MDR-2 E. coli with or without GO-Ag (14 µg ml⁻¹) was cultured in LB broth for 3 h. After that, the bacteria were collected and washed three times with phosphate-buffered saline (PBS, pH 7.4). Next, the bacteria were fixed with 2.5% glutaraldehyde solution, and then the cells were dehydrated by sequential treatment with 50%, 70%, 90% and 100% ethanol for 15 min. Finally, bacteria were transferred to a silicon wafer for gold sputter coating, and then imaged under SEM (Quanta 200FEG, FEI).

Chen Y., Wu W., Xu Z., Jiang C., Han S., Ruan J, & Wang Y. (2020). Photothermal-assisted antibacterial application of graphene oxide-Ag nanocomposites against clinically isolated multi-drug resistant Escherichia coli. Royal Society Open Science, 7(7), 192019.

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Characterization of Sulfur Nanoparticles

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Natural samples were identified using an x-ray diffractometer (X’Pert Pro MPD, PANalytical, The Netherlands) equipped with Cu Kα irradiation (λ = 1.5406 Å). The patterns were recorded from 5° to 85° (2θ) with a scanning speed of 2°/min.
Micro-Raman analysis was carried out on a spectrometer (inVia Reflex, Renishaw, UK) equipped with a 532-nm Nd:YAG laser and a 325-nm He-Cd laser. The beam was focused within 1 μm onto samples at the microscope stage through a ×50 objective.
The morphology of natural S⁰ was observed by environmental SEM (Quanta 200FEG, FEI, USA) equipped with energy-dispersive X-ray spectroscopy detector at 15.0 kV. The colloidal solution in photochemical experiments was collected and dripped onto a holey carbon film supported by a Cu grid. After air-drying, the prepared sample was loaded into the holder of transmission electron microscope (Tecnai F20, FEI, USA) and observed at 200 kV. Digital micrograph version 3.6.5 (Gantan Ltd.) was applied for the image processing.

Li Y., Li Y., Liu Y., Wu Y., Wu J., Wang B., Ye H., Jia H., Wang X., Li L., Zhu M., Ding H., Lai Y., Wang C., Dick J, & Lu A. (2020). Photoreduction of inorganic carbon(+IV) by elemental sulfur: Implications for prebiotic synthesis in terrestrial hot springs. Science Advances, 6(47), eabc3687.

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Scaffold Morphology Analysis by SEM

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Scanning electron microscopy (SEM) was used to investigate the scaffold morphology. Thus, the samples were immersed in MilliQ water at 37 °C for 15 min (maximum swelling); then immediately dropped into liquid nitrogen, physically fractured, and immersed in liquid nitrogen again. Finally, they were freeze-dried. Images of lyophilized scaffolds were obtained by SEM (FEI Quanta 200FEG, Hillsboro, OR, USA) with Schottky’s Filament Field Emission Cannon and voltages of 0.2–30 kV. Micrographs were achieved under ESEM mode at 10 kV and a EDAX Genesis micro-probe (Mahwah, NJ, USA) was used for elemental microanalysis of the scaffold’s different layers.

Campos Y., Sola F.J., Fuentes G., Quintanilla L., Almirall A., Cruz L.J., Rodríguez-Cabello J.C, & Tabata Y. (2021). The Effects of Crosslinking on the Rheology and Cellular Behavior of Polymer-Based 3D-Multilayered Scaffolds for Restoring Articular Cartilage. Polymers, 13(6), 907.

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Comprehensive Characterization of As-Prepared Samples

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The morphological features of the as-prepared samples were characterized using scanning electron microscopy (SEM; Quanta 200 FEG, FEI, America) and transmission electron microscopy (TEM; 200 kV, JEM-100X II, Japan). The specific surface area was analyzed using Brunauer–Emmett–Teller (BET) analysis, and the pore volume and the pore size distribution were calculated using density functional theory (DFT) on a micromeritics instrument (JWBK; 122 W). The degree of defects was measured using Raman spectroscopy (Renishaw in Via). The element valence and element content are displayed through X-ray photoelectron spectra (XPS, XSAM-800, Al Kα, 15 kV).

Huang Y., Wang Y., Cai Y., Wang H., Li Q., Wu Q., Liu K, & Ma Z. (2021). Diatomite waste derived N-doped porous carbon for applications in the oxygen reduction reaction and supercapacitors. Nanoscale Advances, 3(13), 3860-3866.

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