The collected sampls were sliced into smaller pieces and dried at room temperature under shade and then powdered finely and subjected to extraction. Five hundred grams of the powder was macerated with about 2500 ml of 80% methanol for 15 days. The extract was then filtered and the marc was remacerated twice using the same volume of solvent to exhaustively extract the plant material [19 (link)]. The methanol was then removed from the extract by evaporation under reduced pressure using a rota vapor (BUCHI Rotavapour R-200, Switzerland) at 40 °C. The resulting dry hydroalcoholic extract was weighed and calculated for percentage yield, which was 23% (w/w). The dried plant extract was reconstituted with distilled water for oral administration.
Rotavapour r 200
Manufactured by Büchi
Sourced in Switzerland, Germany
The Rotavapour R-200 is a laboratory equipment designed for gentle and efficient solvent evaporation. It features a rotary motion to facilitate the evaporation process and is suitable for a wide range of applications in chemical, pharmaceutical, and research laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Whatman lter, by Cytiva (2 mentions)
Apex 100, by Sartorius (2 mentions)
Whatman no 1 filter paper, by Cytiva (2 mentions)
Ferric chloride, by Thermo Fisher Scientific (1 mentions)
Accu chek performa glucometer, by Roche (1 mentions)
Alloxan monohydrate, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Linomat 5, by CAMAG (1 mentions)
Accu chek advantage, by Roche (1 mentions)
8 protocols using rotavapour r 200
1
Extraction and Yield of Phytochemicals
2
Extraction of Medicinal Compounds from F. sur Leaves
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Five hundred grams of the pounded fresh leaves of F. sur were macerated with 1000 mL of 80% methanol and allowed to stand at room temperature for 72 h using shaker. Seventy-two hours later, the macerate was filtered using Whatman filter paper No. 1. The methanol was then evaporated from the extract under reduced pressure using Rota vapour (BUCHI Rota vapour R-200, Switzerland) at 40°C. The extract obtained was filtered and frozen at −20°C and lyophilized until dried. The yield of the dry extract was found to be 10.5% (w/w).
3
Alloxan-Induced Diabetes Mouse Model
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Alloxan monohydrate (Sigma Chemical Company, USA) was used to induce diabetes in mice and Glibenclamide (Hoechst Pharmaceuticals, Mumbai) was used as a standard hypoglycemic drug. Ethanol (BDH Ltd., England) and distilled water were used for extraction of the plant materials. ACCU CHEK Performa Glucometer (Roche Diagnostics India Pvt. Ltd., India) was used to measure the blood glucose level. For evaporating the solvents, BUCHI Rotavapour R-200, Switzerland and Lyophilizer (freeze dryer) (type: Heto power dry LL3000 Wag tech) was used. The following chemicals were used for phytochemical screening test: Chloroform and Ethyl acetate (ACS, Merck); Hydrochloric acid, Ferric sulphate, Lead acetate and Potassium ferrocyanide (BDH Ltd., England); Petroleum ether 60-80 °C (Labmerk Chemicals LTD India); Sulphuric acid (Farm Italia Carrloerba, Italy); Acetic anhydride and Methanol HPLC grade (Techno Pharmchem, Bahadurgarm, India); n-Hexane (Rathburn Chemicals Ltd., England); Acetonitrile (Sigma Aldrich, Germany) and Ferric chloride (FISHER Scientific Company, USA). All the chemicals were of analytical grades.
4
Extraction and Characterization of Ajuga remota Leaves
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Four hundred gram of the leaves of Ajuga remota was macerated with about 600 ml of 80% methanol for 24 h. The extract was then filtered and the marc was remacerated twice using the same volume of solvent to exhaustively extract the plant material. The methanol was then removed from the extract by evaporation under reduced pressure using a rota vapor (BUCHI Rotavapour R-200, Switzerland) at 40°C. The resulting dry hydroalcoholic extract was weighed and calculated for percentage yield, which was 9.95% (w/w). The dried plant extract was reconstituted with distilled water for oral administration. Thin layer chromatography was run to produce a fingerprint of the 80% methanol extract of the leaves of Ajuga remota and the fingerprint is depicted in Figure 1 .
5
Quantitative Metabolite Analysis via TLC
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TLC aluminum plates silica gel 60F254 [20 × 20 cm, 0.2 mm thickness], TLC scanner 3 with a UV cabinet & Linomat 5, CAMAG, WINCATS Program. ACCU CHEK Performa glucometer (Accu-chek® Advantage, Roche Diagnostic, Mannheim, Germany), Teflon homogenizer (Cole-Parmer, Vernon Hills, IL, USA), light microscope, Buchi Rotavapour R-200, Switzerland, UV-spectrophotometer (Serial No. 340065, Japan), Cotton, pumps, aluminum foil, Whatmann no.1 & 42 filter paper, spectrophotometer (Shimadzu 1200, Japan), and ELISA reader (Humareader Human Company 2106/1682).
6
Extraction of Bioactive Compounds from Aerial Plant Parts
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Maceration technique was used for the extraction of plant material using 80 % methanol. One hundred gram of the aerial parts of the powdered plant material was subjected to maceration process with about 500 ml of 80 % methanol at room temperature for 72 h while shaking occasionally. The extract was then filtered through a muslin cloth and Whatman No.1 filter paper and the marc was remacerated twice using the same volume of solvent to exhaustively extract the plant material. The methanol was then removed from the extract by evaporation under reduced pressure using a rota vapor (BUCHI Rota vapour R-200, Switzerland) at 40 °C. The extract was then dried using a lyophilizer to remove the remaining water. The resulting dry hydroalcoholic extract was weighed and calculated for percentage yield, which was 15.5 % w/w. The dried plant extract was reconstituted with 2 % tween 80 for oral administration.
7
Extraction of Phoenix dactylifera L. Seeds
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The seeds of PDS were collected from Tehama in Yemen 30 kilograms of Phoenix dactylifera L. fruit were removed from the seeds and washed thoroughly with distilled water before being dried by the sun and roasted in an oven (Memmert, Model; SFE600, Schwabach, Germany), at 40-50 C for 4 h. They were then pulverized into a ne powder using a grinder (Taizhou Jincheng Pharmaceutical Machinery Co., Ltd., Model; SF, Jiangsu, China), and soaked in 99.9% methanol (W/V) at room temperature (25C°) for 4 weeks and shaken several times with shaker equipment (GFL 3017 Shaker, Serial No. 103488051, Germany), (maceration method). The extract-containing methanol was ltered with gauze followed by Whatman lter (Whatman, Buckinghamshire, England), and the residue was combined with methanol at a ratio of (W/V). The ltrate was vacuum distilled at 40 °C in a rotary evaporator (Buchi rotavapour R-200 equipped with buchi heating bath B-490, buchi vacuum controller V-800, Germany). The nal extract was a red semi-solid with a dry weight of 90% weighed by balance (Electronic balance, APEX-100, Denver instrument, Germany). This methanol extract (as a whole product).19
8
Extraction of Phoenix dactylifera L. Seeds
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The seeds of PDS were collected from Tehama in Yemen 30 kilograms of Phoenix dactylifera L. fruit were removed from the seeds and washed thoroughly with distilled water before being dried by the sun and roasted in an oven (Memmert, Model; SFE600, Schwabach, Germany), at 40-50 C for 4 h. They were then pulverized into a ne powder using a grinder (Taizhou Jincheng Pharmaceutical Machinery Co., Ltd., Model; SF, Jiangsu, China), and soaked in 99.9% methanol (W/V) at room temperature (25C°) for 4 weeks and shaken several times with shaker equipment (GFL 3017 Shaker, Serial No. 103488051, Germany), (maceration method). The extract-containing methanol was ltered with gauze followed by Whatman lter (Whatman, Buckinghamshire, England), and the residue was combined with methanol at a ratio of (W/V). The ltrate was vacuum distilled at 40 °C in a rotary evaporator (Buchi rotavapour R-200 equipped with buchi heating bath B-490, buchi vacuum controller V-800, Germany). The nal extract was a red semi-solid with a dry weight of 90% weighed by balance (Electronic balance, APEX-100, Denver instrument, Germany). This methanol extract (as a whole product).19
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