The cells of each group in the exponential growth phase were collected and prepared into single cell suspension (1 × 10 4 /ml), then inoculated on 96-well plates with 100 µL cell suspension per well, with 3 replicate wells in each group. Subsequently, 10 µL of CCK-8 solution (Beyotime Biotechnology, Shanghai, China) were added to the wells, and a blank control well only containing the medium and CCK-8 solution was set. After 2 h of incubation, a microplate reader at a wavelength of 450 nm was employed to determine and record the absorbance (A) values of each well. Ultimately, the plate was measured at intervals of 24 h for 5 days.
Cck 8 solution
Manufactured by Beyotime
Sourced in China, United States
The CCK-8 solution is a colorimetric assay used to determine the viability and proliferation of cells. It is a convenient and reliable method for quantifying cell growth and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (91 mentions)
Microplate reader, by Bio-Rad (67 mentions)
Microplate reader, by Agilent Technologies (54 mentions)
96 well plate, by Corning (15 mentions)
Microplate reader, by Molecular Devices (11 mentions)
Cell counting kit 8 (cck8), by Beyotime (6 mentions)
Multiskan go, by Thermo Fisher Scientific (6 mentions)
Multiskan fc, by Thermo Fisher Scientific (6 mentions)
Multiscan mk3, by Thermo Fisher Scientific (5 mentions)
Spectramax m5, by Molecular Devices (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Varioskan flash, by Thermo Fisher Scientific (4 mentions)
Microplate autoreader, by Thermo Fisher Scientific (4 mentions)
Infinite m200, by Tecan (4 mentions)
Elx808, by Agilent Technologies (4 mentions)
Synergy h1, by Agilent Technologies (4 mentions)
Cck 8 kit, by Beyotime (4 mentions)
Cck 8, by Beyotime (4 mentions)
Trizol reagent, by Thermo Fisher Scientific (4 mentions)
Multiskan mk3 microplate reader, by Thermo Fisher Scientific (3 mentions)
773 protocols using cck 8 solution
1
Cell Proliferation Assay with CCK-8
2
Cytotoxicity Assays for JEG3 and HTR8 Cells
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For DMS treatment, JEG3 and HTR8 cells were plated and incubated (37 °C, 5% CO2) in 96-well plates (2 × 103 cells/well) and treated with 2 mM DMS for 24~96 hours. The cultured medium was changed daily until the cells were harvested, and then incubated with CCK-8 solution (C0039, Beyotime Biotechnology) for 2 h, and their absorbance measured at 450 nm. For SDHB siRNA interference, scrambled siRNA or SDHB siRNA were transfected into JEG3 or HTR8 cells using RNAimax (Invitrogen). Transfected cells were then counted and plated and incubated in 96-well plates (2 × 103 cells/well) for 24–96 h followed by CCK-8 solution (C0039, Beyotime Biotechnology) treatment, and their absorbance was measured at 450 nm.
3
Cell Viability Assay with CCK-8
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Each group of cells in logarithmic phase was prepared into a single-cell suspension, and the cell density adjusted at 1,000 cells per well were seeded in a 96-well plate. Following that, six replicate wells were set in each group. On the second day, after the cells were attached, 10 μL of CCK-8 solution (Beyotime Biotechnology) was added to the sample, and a blank control well only containing the medium and CCK-8 solution was set. After incubating for 1 h, a microplate reader at a wavelength of 450 nm was employed to determine and record the absorbance (OD) values of each well. Ultimately, the plate was measured at intervals of 24 h for 5 days.
4
Cell Viability Assay with 5-FU and Doxorubicin
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Cells (2×105/well) were seeded into 96-well plates. 0.1, 1, 3, 5 and 10 µg/ml of 5-FU, or 0.1, 0.3, 0.5, 0.7 and 1.0 µg/ml DOX was added into medium for 24-h culturing. Next, 10 µl CCK-8 solution (Beyotime Institute of Biotechnology) was added to the cell culture for a 2 h co-incubation at 37°C. Absorbance was measured at 450 nm and cell viability was expressed as optical density.
Cells (2×105/well) were seeded into 96-well plates. After 1, 2, 3 or 4-day culturing with or without SB431542, 10 µl CCK-8 solution (Beyotime Institute of Biotechnology) was added to the cell culture for a 2 h co-incubation at 37°C. The mock group was treated with the same volume of DMSO. Absorbance was measured at 450 nm and cell viability was expressed as optical density. Each assay was repeated three times.
Cells (2×105/well) were seeded into 96-well plates. After 1, 2, 3 or 4-day culturing with or without SB431542, 10 µl CCK-8 solution (Beyotime Institute of Biotechnology) was added to the cell culture for a 2 h co-incubation at 37°C. The mock group was treated with the same volume of DMSO. Absorbance was measured at 450 nm and cell viability was expressed as optical density. Each assay was repeated three times.
5
Cell Proliferation Assay Protocol
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Each group of cells in logarithmic phase was prepared into a single‐cell suspension, and the cell density adjusted at 1,000 cells per well were seeded in a 96‐well plate. Following that, six replicate wells were set in each group. On the second day, after the cells were attached, 10 μL of CCK‐8 solution (Beyotime Biotechnology) was added to the sample, and a blank control well only containing the medium and CCK‐8 solution was set. After incubating for 1 hr, a microplate reader at a wavelength of 450 nm was employed to determine and record the absorbance (OD) values of each well. Ultimately, the plate was measured at intervals of 24 hr for 5 days.
6
Cytotoxicity Assay of C-33A and HeLa Cells
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C-33A and HeLa CC cells in the exponential growth phase were taken and made into a single-cell suspension. After being counted, cell density was adjusted (1000 cells per well). We inoculated them in 96-well plates (6 replicates in each group, 6 well plates in total) and cultured for 12 h, 24 h, 48 h, 72 h and 96 h. After adherent culture, with the addition of 90 µL medium and 10 µL CCK-8 solution (Beyotime, Shanghai, China) into the samples, blank control wells containing only CCK-8 solution and medium were set. After a 2-hour incubation, the absorbance (A) value of each well was evaluated and recorded with a Microplate Reader at the wavelength of 450 nm.
7
Cytotoxicity Assay of Fe3O4@PDA Nanoparticles on MSCs
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We inoculated 5×104 MSCs into 96-well plates and cultured them with different concentrations of Fe3O4@PDA NPs (0, 25, 50, 75, 100, 200 μg/mL) for 24 h. We added 10 ul CCK-8 solution (Beyotime Biotechnology, China) to each well and incubated it at 37 °C for 2 h. The absorbance at 450 nm was detected using a microplate reader. The test was repeated thrice at each concentration.
We inoculate 5×104 MSCs and 5×104 Fe3O4@PDA-labeled MSCs (50 μg/mL) into 96-well plates, respectively. They were subsequently cultured at 37 °C for 1, 3, 5, and 7 days. Next, 10 ul CCK-8 solution (Beyotime Biotechnology, China) was added to each well and incubated at 37 °C for 2 h. Finally, the absorbance at 450 nm was detected using a microplate reader with triplicate experiments being conducted for each group.
We inoculate 5×104 MSCs and 5×104 Fe3O4@PDA-labeled MSCs (50 μg/mL) into 96-well plates, respectively. They were subsequently cultured at 37 °C for 1, 3, 5, and 7 days. Next, 10 ul CCK-8 solution (Beyotime Biotechnology, China) was added to each well and incubated at 37 °C for 2 h. Finally, the absorbance at 450 nm was detected using a microplate reader with triplicate experiments being conducted for each group.
8
Cell Viability Assay Protocol
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Each group of cells was adjusted at 1,000 cells per well. 10 mL of CCK-8 solution (Beyotime Biotechnology, Shanghai, China) was added to the cell dish after 24 hours, and a blank control has only CCK-8 solution. Absorbance (OD) value of each well was read for each well at 450 nm and tested every 24 hours for three days.
9
Cell Proliferation Kinetics Assay
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C-33 A and HeLa CC cells in the exponential growth phase were taken and made into a single-cell suspension. After being counted, cell density was adjusted as 1000 cells per well. We inoculated them in 96-well plates (6 replicates in each group, 6 well plates in total) and cultured for 12 h, 24 h, 48 h, 72 and 96 h. After adherent culture, with the addition of 90 µL medium and 10 µL CCK-8 solution (Beyotime, Shanghai, China) into the samples, blank control wells containing only CCK-8 solution and medium were set. After a 2-h incubation, the absorbance (A) value of each well was evaluated and recorded with Multiskan FC Microplate Reader (Thermo Fisher, MA, USA) at the wavelength of 450 nm.
10
Cytotoxicity and IC50 Evaluation of Docetaxel
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For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA).
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