Phosphoric acid rinse solution

Manufactured by Beyotime

Sourced in China

Phosphoric acid rinse solution is a laboratory product designed to clean and condition surfaces. It is a dilute solution of phosphoric acid that helps remove deposits and prepare surfaces for subsequent treatments.

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Lab products found in correlation

Acetone, by Beyotime (2 mentions) Glutaraldehyde, by Sinopharm (2 mentions) Osmium tetroxide, by Absin (1 mentions)

3 protocols using phosphoric acid rinse solution

Ultrastructural Analysis of Rat Myocardium

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The myocardial tissue of rats in each group was cut into 50- to 100-nm-thick sections and fixed with 2.5% glutaraldehyde (China National Medicine Group Chemical Reagent Co., Ltd.) before operation, then fixed with 1% osmium tetroxide(Absin Bioscience Inc. Shanghai China), rinsed with Phosphoric acid rinse solution (Beyotime Institute of Biotechnology, Shanghai, China),stained with 3% uranium dioxide acetate and lead nitrate, and observed the ultrastructure of the tissue under TEM.

Li Y., Liu M., Song X., Zheng X., Yi J., Liu D., Wang S., Chu C, & Yang J. (2020). Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy. Frontiers in Pharmacology, 11, 1150.

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Ultrastructural Analysis of Myocardial Tissue

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Left ventri cular myocardial tissue samples were cut into slices and fixed with 2.5% glutaraldehyde (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), post-fixed with 1% osmium tetraoxide (Absin Bioscience Inc., Shanghai, China), rinsed with phosphoric acid rinse solution (Beyotime Institute of Biotechnology), dehydrated using a series of acetone (Beyotime Institute of Biotechnology) at different concentrations, embedded and solidified, and cut into slices (thickness, 50-100 nm). The ultrathin slices were stained using 3% uranyl acetate (Shanghai Fortune Biological Technology Co., Ltd., Shanghai, China) and lead nitrate (Tanyun Industry Fine Chemical Co., Ltd., Yingkou, China) at 37°C for 30 min. Samples were observed under a transmission electron microscopy and the images were saved.

Liang B., Xiao T., Long J., Liu M., Li Z., Liu S, & Yang J. (2017). Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy. International Journal of Molecular Medicine, 40(6), 1781-1791.

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Ultrastructural Analysis of Myocardial Tissue

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The myocardial tissue was firstly fixed with 2.5% glutaraldehyde (Sinopharm Chemical Reagent Co., Ltd.). Then it was sequentially fixed with 1% osmium tetroxide (Absin Bioscience Inc., Shanghai, China), rinsed with Phosphoric acid rinse solution (Beyotime Institute of Biotechnology, Shanghai, China), dehydrated at different concentrations of acetone (Beyotime Institute of Biotechnology, Shanghai, China), and solidified into 50-100 nm thick slices. After being stained with 3% uranyl acetate (Johnson Biotechnology Co., Ltd., Shanghai, China) and lead nitrate (Tianyuan Industrial Fine Chemical Co., Ltd., Yingkou, China), the ultrathin slices were observed under TEM.

Liu M., Li Z., Liang B., Li L., Liu S., Tan W., Long J., Tang F., Chu C, & Yang J. (2018). Hydrogen sulfide ameliorates rat myocardial fibrosis induced by thyroxine through PI3K/AKT signaling pathway. Endocrine journal, 65(7).

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