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Annexin 5 fluorescein isothiocyanate propidium iodide apoptosis kit

Manufactured by BD
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The Annexin V-fluorescein isothiocyanate-propidium iodide apoptosis kit is a laboratory tool used to detect and quantify apoptosis, a specific type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a DNA-binding dye, to differentiate between viable, apoptotic, and necrotic cells.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit Annexin V-fluorescein isothiocyanate (V-FITC)/propidium iodide (PI) apoptosis kit Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Assay Kit Annexin-V Fluorescein Isothiocyanate and Propidium Iodide Apoptosis Detection Kit Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis kit Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit Annexin V-fluorescein isothiocyanate/propidium iodide Annexin V/propidium iodide (PI) apoptosis kit Annexin V-fluorescein isothiocyanate apoptosis kit

2 protocols using annexin 5 fluorescein isothiocyanate propidium iodide apoptosis kit

1

Annexin V-FITC Apoptosis Assay

Cited 1 time
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Apoptosis of A549 and H1299 cells was examined by the Annexin V-fluorescein isothiocyanate-propidium iodide apoptosis kit (BD Biosciences, NJ, USA). Cells were collected after transfection and washed with cold PBS. The cells were then suspended with 1 × binding buffer (500 μl), mixed with 5 μl Annexin, and preserved at room temperature for 15 min away from light with V‐FITC and 5 μl PI. The percentage of apoptotic cells was assessed on an FACS Calibur flow cytometer [25 (link)].
Li S., Qiu C., Sun D., Yang S, & Wang L. (2024). circNINL facilitates aerobic glycolysis, proliferation, invasion, and migration in lung cancer by sponging miR-3918 to mediate FGFR1 expression. European Journal of Medical Research, 29, 67.
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2

Annexin V-FITC/PI Apoptosis Assay

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The cell apoptosis rate was measured by using an annexin V‐fluorescein isothiocyanate/propidium iodide apoptosis kit (BD Biosciences) according to the manufacturer's instructions. We analysed the cellular fluorescence with the FACSCalibur™ flow cytometer (BD Biosciences). Three technical replicates were applied for each sample.
Peng K., Chen W., Xia F., Liu H., Meng X., Zhang J., Liu H., Xia Z, & Ji F. (2019). Dexmedetomidine post‐treatment attenuates cardiac ischaemia/reperfusion injury by inhibiting apoptosis through HIF‐1α signalling. Journal of Cellular and Molecular Medicine, 24(1), 850-861.
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