Human tnf α elisa kit

Manufactured by MultiSciences Biotech

Sourced in China

The Human TNF-α ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human tumor necrosis factor-alpha (TNF-α) levels in various biological samples, such as cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Human il 6 elisa kit, by MultiSciences Biotech (4 mentions) Human il 1β elisa kit, by MultiSciences Biotech (2 mentions) Reverse transcription kit, by Takara Bio (1 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Mcc950, by AbMole (1 mentions) Pro caspase 1, by ABclonal (1 mentions) Caspase 1, by ABclonal (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions) 3 methyladenine 3 ma, by Merck Group (1 mentions) Sybr premix ex taq, by Takara Bio (1 mentions) Dcfh da, by Beyotime (1 mentions) Nlrp3, by ABclonal (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by ABclonal (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Ifn γ elisa kit, by MultiSciences Biotech (1 mentions) Cytotoxicity detection kitplus, by Merck Group (1 mentions) Fam labeled pd l1 antibody, by BioLegend (1 mentions) Elx800, by Agilent Technologies (1 mentions) Human ifn γ elisa kit, by MultiSciences Biotech (1 mentions)

Spelling variants of this product

TNF-α (28 citations) Human TNF-α (2 citations) ELISAs (2 citations)

7 protocols using human tnf α elisa kit

Ferulic Acid Modulates Inflammatory Response

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RPMI-1640 culture medium and foetal bovine serum were obtained from Gibco. Ferulic acid (FA), lipopolysaccharide (LPS), Phorbol-12-myristate-13-acetate (PMA), thiazolyl blue tetrazolium bromide (MTT), and 3-methyladenine (3-MA) were purchased from Sigma-Aldrich. MCC950 was purchased from Abmole Bioscience Inc. DCFH-DA was purchased from Beyotime Biotechnology Co., Ltd. The reverse transcription kits and SYBR Premix Ex Taq were purchased from Takara Biomedical Technology. The antibodies against Microtubule Associated Protein 1 Light Chain 3 Beta (LC3), Sequestosome 1 (p62), Beclin-1, NLRP3, pro-caspase-1, caspase-1, pro-Interleukin 1 Beta (IL-1β), IL-1β and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were purchased from ABclonal Technology. The Human IL-6 ELISA Kit, Human IL-1β ELISA kit and human TNF-α ELISA Kit were purchased from Multisciences (Lianke) Biotech, Co., Ltd. All other reagents used were obtained from local commercial sources.

Liu Y., Shi L., Qiu W, & Shi Y. (2022). Ferulic acid exhibits anti-inflammatory effects by inducing autophagy and blocking NLRP3 inflammasome activation. Molecular & Cellular Toxicology, 18(4), 509-519.

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Cytotoxicity and Immune Response Profiling

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The quantified levels of IFN-γ, TNF-α, Granzyme B secreted by co-cultured activated CD8⁺ T cells were detected using ELISA kits, including Human TNF-α ELISA Kit, IFN-γ ELISA Kit (MultiSciences, China) and Human Granzyme B ELISA Kit (Beyotime Institute of Biotechnology) according to the manufacturer’s protocol. The supernatants were collected and detected by 450 nm Optical densities using microplate reader (Bio-Tek). For the surface PD-L1, ~ 80% confluency BC cells were stained by FAM-labeled PD-L1 antibody (BioLegend, USA). After washing by PBS three times, FAM-positive cells were detected by flow cytometry. The release of lactate dehydrogenase (LDH) in the supernatants was detected on cell lysis using the Cytotoxicity Detection Kit PLUS (Sigma-Aldrich) according to the manufacturer’s protocol.

Zhang Z., Huo W, & Li J. (2024). circATAD2 mitigates CD8+ T cells antitumor immune surveillance in breast cancer via IGF2BP3/m6A/PD-L1 manner. Cancer Immunology, Immunotherapy : CII, 73(7), 130.

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Quantifying Cytokine Production by ELISA

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To measure the levels of TNF-α and IFN-γ produced by cells, the supernatants were collected and the concentrations of TNF-α and IFN-γ were measured by the Human TNF-α ELISA Kit (MultiSciences, Hangzhou, China) and Human IFN-γ ELISA Kit (MultiSciences) according to the manufacturer’s guidelines. Optical densities were determined using a microplate reader (Bio-Tek Elx 800; Bio-Tek Instruments) at 450 nm.

Chen Z.Q., Zuo X.L., Cai J., Zhang Y., Han G.Y., Zhang L., Ding W.Z., Wu J.D, & Wang X.H. (2023). Hypoxia-associated circPRDM4 promotes immune escape via HIF-1α regulation of PD-L1 in hepatocellular carcinoma. Experimental Hematology & Oncology, 12, 17.

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Urinary Biomarker Analysis in Kidney Injury

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Following centrifugation of urine for 20 min, the supernatant was collected for ELISA assay. Mouse Urinary Albumin (UALB) ELISA Kit was used to measure the UALB. Urine neutrophil gelatinase-associated lipocalin (NGAL) was detected by Mouse NGAL ELISA Kit. We performed the detection of urinary kidney injury molecule 1 (KIM-1) using mouse KIM-1 ELISA Kit. The three ELISA kits were purchased from Wuhan Fine Biotech (Wuhan, China).
The levels of interleukin-6 (IL-6), IL-1β, tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) in renal cortex and HK2 cells were detected according to ELISA kit instructions. The kits used in this part were purchased from MULTI SCIENCES (Zhejiang, China) and they included Mouse IL-6 ELISA kit, Human IL-6 ELISA kit, mouse IL-1β ELISA kit, human IL-1β ELISA kit, mouse TNF-α ELISA kit, human TNF-α ELISA kit, mouse MCP-1 ELISA kit and Human MCP-1 ELISA Kit.

Li X.J., Liu T, & Wang Y. (2023). Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway. Journal of Natural Medicines, 78(1), 53-67.

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Cytokine Levels Under SFP and DSFP-45

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The effects of SFP and DSFP-45 on the levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, were studied by ELISA. The level of IL-1β was measured using the Human IL-1β ELISA Kit (EHC002b.48, NeoBioscience Technology Co., Ltd., Shenzhen, China) according to the manufacturer’s protocol. The content of IL-6 was determined using the Human IL-6 ELISA Kit (70-EK106/2-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China). The amount of TNF-α was detected by the Human TNF-α ELISA Kit (70-EK182-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China).

Yao W., Yong J., Lv B., Guo S., You L., Cheung P.C, & Kulikouskaya V.I. (2023). Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment. Marine Drugs, 21(8), 430.

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Cytokine and Receptor ELISA Quantification

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sB7-H5 and cytokines were detected using ELISA following the Manufacturer's manual. The Human Platelet receptor Gi24 (C10orf54) ELISA kit (CSB-EL002961HU, Cusabio technology, Wuhan, Hubei, China), Human IL-6 ELISA Kit (70-EK106/2-96, MultiSciences Biotech, Hangzhou, Zhejiang, China), Human IL-10 ELISA Kit (70-EK110/2-96, MultiSciences Biotech, Hangzhou, Zhejiang, China), and Human TNF-α ELISA Kit (70-EK182-96, MultiSciences Biotech, Hangzhou, Zhejiang, China) were used.

Xu R., Gong J., Chen W., Jin Y, & Huang J. (2021). Soluble B7-H5 Is a Novel Diagnostic, Severity, and Prognosis Marker in Acute Pancreatitis. BioMed Research International, 2021, 1223850.

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Quantifying Cytokine and Perforin Levels

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To measure the production of perforin, TNF-α, CXCL9, CXCL10 and CXCL11, the supernatant was collected and centrifuged to remove remaining cells. The levels of perforin and the above cytokines were evaluated by human perforin ELISA Kit (Enzyme-linked Biotechnology Co. Ltd., Shanghai, China), human TNF-α ELISA Kit (Multisciences, Hangzhou, China), Human CXCL9 ELISA Kit (Multisciences, Hangzhou, China), Human CXCL10 ELISA Kit (Multisciences, Hangzhou, China), and Human CXCL11 ELISA Kit (Multisciences, Hangzhou, China) according to the manufacturers’ instructions, respectively. The ODs were measured at 450 nm and the concentrations of perforin and the cytokines were determined according to their corresponding standard curves.

Luo H., Zhou Y., Zhang J., Zhang Y., Long S., Lin X., Yang A., Duan J., Yang N., Yang Z., Che Q., Yang Y., Guo T., Zi D., Ouyang W., Yang W., Zeng Z, & Zhao X. (2023). NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions. Frontiers in Immunology, 13, 1087689.

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