Wallac wizard 1480 wizard

For the biodistribution study, eight mice were injected i.v. with ¹²⁵I-labelled rA1M (5 mg/kg) and eight mice were injected i.v. with ¹²⁵I-labelled A1M (5 mg/kg) and the positively charged amino acid solution, Vamin (Vamin 18N/l, Fresenius Kabi, Sweden, 35 mg/200 µL, administered i.p.). Four mice of each group were sacrificed after 10 min and the other four after 60 min. Kidneys, livers, spleens, and femurs were collected in scintillation tubes, weighed, and measured in a Nal (TI) well counter (Wallac Wizard 1480 Wizard, PerkinElmer). Results were corrected for background and radioactive decay. Organ specific uptake values were calculated as percent injected activity per gram of tissue (%IA/g).

Biodistribution studies were conducted to evaluate the uptake of ¹¹¹In-DTPA-11B6 in human prostate cancer LNCaP xenografts. Mice (n = 3 to 5 per time point) received ¹¹¹In-DTPA-11B6 (0.4 to 0.6 MBq, 20 μg of mAb, in approximately 100 μL of PBS) through intravenous (i.v.) tail vein injection. Blood and organs (including tumour) were taken at 4, 24, 48, 72 and 168 h post-injection, weighed and measured in a NaI(TI) well counter (Wallac Wizard 1480 Wizard, Perkin Elmer). The activity injected into each animal was measured and used to determine the count rate, in comparison with a standard solution of ¹¹¹In-DTPA-11B6. Data were corrected for background and physical decay.
Organ-specific uptake values were calculated as percent injected activity per gram of tissue (%IA/g) or percent injected activity (%IA). Among the organs resected were the lateral and ventral prostate, from now on referred to as prostate, and the submandibular glands, from now on called salivary glands.