Mp e 65 mm macro lens

All fossil specimens were documented on a Keyence VHX-6000 digital microscope. Extant specimens were either documented on the same microscope or with a super-macro-photography set up.
Fossil specimens recorded on the VHX microscope were documented with different illumination settings, low-angle ring light and cross-polarised co-axial light, and in front of a white and a black background [17 (link)]. Images with the highest degree of detail were used for further analysis.
The super-macro-photography set up included a Canon EOS 650D camera with an MP-E 65 mm macrolens. Illumination was either provided by macro-twin flashes or a pair of two single flashes (Yongnuo Digital Speedlite YN560EX II). Polarisers were placed on flashes and in front of the lens to provide cross-polarised light [57 ,58 ].
All images are composite images [59 (link)]; a stack of several frames of shifting focal planes was recorded for each image detail (processed with CombineZP or built-in software of microscope); adjacent images details were recorded (each with a stack) and merged to a large panorama (processed with Adobe Photoshop CS3, Adobe Photoshop Elements 11, or built-in software of microscope). Images recorded on VHX microscope are HDR images, additionally. The further processing of images (contrast, levels, and sharpness) was performed in Adobe Photoshop CS2.

The specimens CNU-PHA-NN2012001, CNU-PHA-NN2012002 and CNU-PHA-NN2012003 were examined under dry conditions using a Leica DCF 500 dissecting microscope and illustrated with the aid of a drawing tube. Photographs were taken using a Canon EOS 550D digital camera coupled to a Canon 50 mm macro lens (and an extension tube as appropriate), or a Canon MP-E 65 mm macro lens (all lenses equipped with polarizing filters).

The macrophotograps of Figs. 1c–l,n–u, 2q–u were taken with a Keyence digital microscope (VHX-700F, objective VH-Z20T and at the Institute of Biology Education (Zoology), University of Cologne. The specimens were not submerged in isopropanol in order to retain the contrasts of the edges. The pictures were processed and arranged to figures with Adobe Photoshop CS3 and Adobe Photoshop Elements.
The macrophotographs of Fig. 2a–d were taken with the specimens submerged in isopropanol using a Canon EOS 600D SLR camera equipped with a Canon MP-E 65 mm macro lens. Free image stacking software (CombineZM by Alan Hadley) was then employed to produce composites with enhanced depth of field using photographs with differing focal planes. These were processed and arranged into figures using Adobe Photoshop CS3.
The SEM photographs were taken at the Biocentre Cologne, (FEI -Quanta FEG 250, Thermo Fischer Scientific).
The eurypterid eyes figured in this contribution are stored in the Geological Institute of the University of Cologne, leg. E. Evangelou (Figs. 1c–e,q–u, 2). They were collected from Siegenian (possibly lowermost Emsian in terms of the international stratigraphic frame) strata of the Jaeger Quarry near Frielingshausen/Bergisches Land, Germany.

The following abbreviations used in the text refer to the examined collections:
BMNH Natural History Museum, London, United Kingdom;
HBUM Hebei University Museum, Baoding, China;
MNHN Muséum National d’Histoire Naturelle, Paris, France.
Figures of antennal morphological details were drawn by hand, using a Nikon SMZ1500 stereomicroscope equipped with a camera lucida. Photographs of other morphological details were taken using a Leica M205A stereomicroscope equipped with a Leica DFC450 camera which was controlled using the Leica application suite 4.3. Habitus were taken with a Canon EOS 5D Mark III camera connected to a Canon MP-E 65 mm macro lens.