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35 protocols using coreldraw x8

1

Colocalization Analysis in Immunocytochemistry

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Colocalization in ICC samples was measured by using ImageJ software and Pierson’s Coefficient was calculated and detailed colocalization analysis were performed with the use of Coloc2 Plugin from Fiji (ImageJ software). Figures were composed with CorelDRAW X8 (Corel Corporation, Ottawa, Canada) or with Microsoft Power Point (Microsoft Corporation).
Statistical analysis was performed using Microsoft Excel (Microsoft Corporation) and GraphPad PRISM 5 (GraphPad Software, San Diego, CA, USA). Images were processed with ImageJ V1.41, NIH, USA and/or CorelDRAW X8 (Corel Corporation, Ottawa, Canada).
Statistical tests performed were Student’s-two-tailed t-test, one-way-Analysis of Variance (ANOVA) and repeated-measures ANOVA for grouped analysis, followed by Tukey’s post-hoc tests for multiple comparison.
Data were expressed as mean ± SEM and a 0.5% general significance level was defined, with significance levels as follows: *: p < 0.05; **: p < 0.01; ***: p < 0.001.
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2

Statistical Analysis of Conditioned Responses and Biochemical Data

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Data was expressed as mean ± SEM. Statistical differences between percentages of CRs were determined by repeated measures two-way ANOVA, followed by Tukey’s post-hoc analysis. Biochemical data were analyzed with three-way ANOVAs (using training, sessions, and hippocampal side as fixed factors), followed by Tukey’s post-hoc analysis. Statistical significance was set at p < 0.05. If the Levene’s test for normal distribution was significant then data were normalized by square root transformation (percentage of conditioned responses). All analyses were performed using the SPSS software v.28 (RRID:SCR_002865; IBM, US) and final figures were prepared using CorelDraw X8 Software (RRID:SCR_014235; Corel Corporation, Canada).
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3

Statistical Analysis of Biological Experiments

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Data was represented as the mean ± SEM and analyzed by three- or two-way ANOVA, followed by Tukey’s post-hoc analysis. When comparing only two groups, Student t test was used. Statistical significance was set at p < 0.05. All analyses were performed using SPSS software v.24 (RRID:SCR_002865; IBM, USA) and GraphPad Prism software v.8.3.1 (RRID:SCR_002798; Dotmatics, USA). Final figures were prepared using CorelDraw X8 Software (RRID:SCR_014235; Corel Corporation, Canada).
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4

Fabrication of Paper Microfluidic Devices

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All paper microfluidic devices used in the current work were fabricated on Whatman® qualitative filter paper (Grade 4, thickness 0.205 mm, Cytiva, India). All the paper-based devices used in this work were designed using the CorelDraw X8 software (Corel Corporation, Ottawa, Canada). The design was printed using a wax printer (ColorQube 8570, Xerox India Ltd., India).
The printed devices were kept on the hot plate at 120°C for 2 minutes to melt the wax so that it can percolate down to the other side of the paper and forms the hydrophobic barrier. The printed wax devices were then cut using a computer-controlled laser cutting and engraving machine (Model CMA 6040, GD Han's Yueming Laser Group Co., Ltd, China) or with simple scissors for further use. All the paper-based devices used in this work are used without any further modifications.
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5

Optimizing Visualization with CorelDRAW and R

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Figure preparation: CorelDRAW x8 (Corel); Bioinformatic analyses: R v 4.0.3 (R Foundation for Statistical Computing).
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6

Image Analysis with CorelDRAW and ImageJ

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Figure preparation: CorelDRAW X8 (Corel, Ottawa, ON, Canada); Image analysis: ImageJ 1.52h (NIH, Bethesda, MD, USA); statistical analysis: MATLAB R2018a (MathWorks, Natick, MA, USA).
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7

Evaluation of Mandibular Molar Root Canal Curvature

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The study was approved by the Marmara University Health Sciences Ethics Committee (protocol no. 22-2016). Sixty extracted mandibular first molars without resorption, crack, fracture or restoration on the root surface were selected. All tissue remnants and debris on the root surface were removed with ultrasonic tips, and all of the crowns were removed. Radiological images were obtained with the paralleling technique in the buccolingual and mesiodistal plane. Radiographic images were transferred to the CorelDRAW X 8 (CorelDRAW Graphics Suite X 8-Corporate License, 2016, USA) program. According to Schneider's method (Schneider, 1971 (link)), the degree of curvature was calculated with the same software. The 60 mandibular first molars with mesial root canal curvatures greater than 25° were included.
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8

Statistical Analysis of Experimental Data

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For statistical analysis, GraphPad Prism v8 (Graphpad Software, San Diego, CA, USA) (Student’s t-test, Grubb’s outlier test, Kruskal–Wallis H-tests, Chi-squared test with Yates correction) and the Python v3.7 (Python Software Foundation, Beaverton, OR, USA) packages Matplotlib, Numpy, SciKit, Pandas, Requests, and Statsmodel were used as appropriate. The level of statistical significance was predefined at p < 0.05 for all statistical tests and corrected for multiple comparisons where appropriate.
For a graphical representation of the data, GraphPad Prism v8 and the Python 3.7 packages Seaborn, Matplotlib, Numpy, NetworkX, and Pandas were applied. Graphical post-processing was performed using CorelDraw X8 (Corel Corporation, Ottawa, ON, Canada).
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9

Phylogenetic Analysis of Nucleotide Sequences

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The best-fitting models for nucleotide substitution selected by jModelTest software43 (link) using the Akaike information criterion was GTR + I + G. Phylogenies were reconstructed for each alignment under Bayesian inference (BI) using MrBayes v. 3.244 (link). and Maximum Likelihood (ML) using PhyML45 (link) with 1000 replicates. The BI was run using four Markov chain Monte Carlo searches with 10,000,000 generations and sampling tree topologies every 100 generations. The burning was set to the first 30% of generations; the consensus trees were estimated using the remaining topologies. The nodes with posterior probabilities and bootstrap values greater than 0.95/65 were considered valid. The final trees were edited using CorelDRAW X8. Pairwise genetic distances within and among sequences were calculated using the Kimura-2-parameter (K2P) model and a bootstrap procedure with 1000 replicates in Geneious 7.1.338 (link).
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10

Multivariate Analysis of Molecular Fingerprints

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Principal component analysis (PCA) and multi-dimensional scaling (MDS) were used as alternative grouping methods, producing three-dimensional plots in which the entries were spread according to their relatedness. Dimensioning techniques were executed with AFLP data after conversion into a band matching table. Automated band matching was performed on all fingerprint entries within the comparison, considering minimum profiling of 5%, with the optimization and position tolerances for selecting bands set to 0.10%. Default settings were applied for PCA and MDS in BioNumerics v7.6, subtracting the average for characters [38 (link)]. All Figs were exported and treated using Corel Draw X8.
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